Quasi-Elastic Light Scattering Studies of Protein Refolding and Aggregation

重组DNA产生的杂合蛋白 技术经常孤立地展开和不活跃 forms. 这些蛋白质必须重新折叠， 产品获得。 在这篇文章中，一部小说 准电光散射（QELS）技术， 建议用于研究蛋白质重折叠， 聚合来 促进蛋白质的加工条件 重折叠和环境对功能的影响 折叠蛋白质的性质将使用 β-半乳糖苷酶作为模型蛋白。

Heterologous proteins produced by recombinant DNA technology are frequently isolated in unfolded and inactive forms. These proteins must be refolded before a useful product is obtained. In this proposal, a novel quasi-electric light scattering (QELS) technique is proposed for use in studying protein refolding and aggregation. Processing conditions that facilitate protein refolding and environmental effects on the functional properties of the folded proteins will be evaluated using beta-galactosidase as a model protein.