Quasi-Elastic Light Scattering Studies of Protein Refolding and Aggregation

通过重组DNA技术生产的异源蛋白经常以未折叠和非活性的形式分离出来。在获得有用的产品之前，这些蛋白质必须重折叠。在这个方案中，提出了一种新的准电光散射(QELS)技术用于研究蛋白质的折叠和聚集。将以β-半乳糖苷酶作为模型蛋白质，评估促进蛋白质复性的加工条件和环境对折叠蛋白质功能性质的影响。

Heterologous proteins produced by recombinant DNA technology are frequently isolated in unfolded and inactive forms. These proteins must be refolded before a useful product is obtained. In this proposal, a novel quasi-electric light scattering (QELS) technique is proposed for use in studying protein refolding and aggregation. Processing conditions that facilitate protein refolding and environmental effects on the functional properties of the folded proteins will be evaluated using beta-galactosidase as a model protein.