Quasi-Elastic Light Scattering Studies of Protein Refolding and Aggregation

蛋白质重折叠和聚集的准弹性光散射研究

• 批准号: 9009838
• 负责人: Shang-Tian Yang
• 金额: $ 8万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9009838&HistoricalAwards=false
• 关键词: Quasi; Elastic; Light; Scattering; Studies

Heterologous proteins produced by recombinant DNA technology are frequently isolated in unfolded and inactive forms. These proteins must be refolded before a useful product is obtained. In this proposal, a novel quasi-electric light scattering (QELS) technique is proposed for use in studying protein refolding and aggregation. Processing conditions that facilitate protein refolding and environmental effects on the functional properties of the folded proteins will be evaluated using beta-galactosidase as a model protein.

重组DNA技术产生的异源蛋白经常以未折叠和无活性的形式分离出来。这些蛋白质必须重新折叠才能得到有用的产物。本文提出了一种新的准电光散射（QELS）技术，用于研究蛋白质的再折叠和聚集。将以-半乳糖苷酶为模型蛋白，评估促进蛋白质重折叠的加工条件和环境对折叠蛋白质功能特性的影响。