Quasi-Elastic Light Scattering for in-depth protein and protein-complex characterization

四位申请人（Stetefeld博士、McKenna博士、O 'Neil博士和Mark博士）实验室的研究项目集中于研究各种有趣的生物系统，包括完整的膜蛋白通道、极端嗜S层组分、病毒核酸识别蛋白和调节性细菌激酶。连接每个研究小组的共同特征是特定蛋白质样品及其潜在配体的结构和功能表征，以研究它们在调节感兴趣的生物系统中的作用。结构生物学领域的快速发展使本提案中包括的研究小组能够确定蛋白质-配体复合物的高分辨率三维图像，并开始根据决定相互作用的关键分子特征来表征其生化功能和机制。

Research programs in the laboratories of the four applicants (Drs. Stetefeld, McKenna, O'Neil and Mark) are focused on investigating a variety of interesting biological systems including integral membrane protein channels, extremophilic S-layer components, viral nucleic acid recognition proteins and regulatory bacterial kinases. The common feature that links each of these research groups is the structural and functional characterization of specific protein samples and their potential ligands to investigate their role in regulating the biological systems of interest. Rapid advances in the field of structural biology have allowed the research groups included in this proposal to determine high-resolution three-dimensional images of protein-ligand complexes, and begin to characterize their biochemical function and mechanism in terms of key molecular features that dictate the interaction.