Core Instrumentation in Molecular Biology for the Departmentof Biological Sciences

将建立一个仪器核心，以促进和促进分子生物学和分子遗传学的各种和正在进行的一系列项目。所要求的新设备包括：DNA测序仪和用于DNA/蛋白质序列分析的扩展的中央计算机系统。该设施还将包括一台DNA合成器、一台荧光仪、一台高效液相色谱仪和一台高压纸电泳仪。该设施将由一名专门的技术人员监督和维护，他还将进行DNA合成和DNA测序凝胶运行。该设施的主要用户是研究二氢叶酸还原酶基因在培养的哺乳动物细胞中的表达，增强子在免疫球蛋白基因调控中的作用，RNA剪接和3'端形成的机制，酵母线粒体的生物发生，大肠杆菌的转录终止和反终止。线虫细胞分化调控基因的研究。该设施将向哥伦比亚大学生物科学系的所有24个实验室开放，并在一定程度上向本科生开放。

A core of instrumentation will be established to facilitate and promote a variety and ongoing series of projects in molecular biology and molecular genetics. The following new equipment items requested are: a DNA sequencer, and an expanded centralized computer system for DNA/protein sequence analysis. The facility will also include a DNA synthesizer, a fluorimeter, an HPLC, and a high voltage paper electrophoresis apparatus. The facility would be supervised and maintained by a dedicated technician, who would also carry out the DNA syntheses and the DNA sequencing gel runs. Major users of this facility are studying the expression of the dihydrofolate reductase gene in cultured mammalian cells, the role of the enhancer in immunoglobulin gene regulation, the mechanism of RNA splicing and 3' end formation, biogenesis of mitochondria in yeast, transcription termination and antitermination in E. coli, and the characterization of genes controlling cell differentiation in C. elegans. The facility would be available to all 24 laboratories of the Columbia Department of Biological Sciences, and to a limited extent, to undergraduates.