Core Instrumentation in Molecular Biology for the Departmentof Biological Sciences

将建立一个仪器核心， 促进各种和正在进行的一系列项目，在分子 生物学和分子遗传学。 以下新设备 要求的项目是：一个DNA测序仪，和一个扩展的 用于DNA/蛋白质序列分析的中央计算机系统。 该设施还将包括一个DNA合成器，一个荧光计， HPLC和高压纸电泳仪。 的 设施将由一个专门的 技术人员，谁也将进行DNA合成和 DNA测序凝胶运行。 该设施的主要用户是 研究二氢叶酸还原酶基因在大肠杆菌中的表达， 培养的哺乳动物细胞，增强子在 免疫球蛋白基因调控，RNA剪接机制， 3 '末端形成，酵母中线粒体的生物发生， E.大肠杆菌， 在C. 优雅 该设施将供所有24个实验室使用 哥伦比亚生物科学系的教授， 在一定程度上，对本科生来说。

A core of instrumentation will be established to facilitate and promote a variety and ongoing series of projects in molecular biology and molecular genetics. The following new equipment items requested are: a DNA sequencer, and an expanded centralized computer system for DNA/protein sequence analysis. The facility will also include a DNA synthesizer, a fluorimeter, an HPLC, and a high voltage paper electrophoresis apparatus. The facility would be supervised and maintained by a dedicated technician, who would also carry out the DNA syntheses and the DNA sequencing gel runs. Major users of this facility are studying the expression of the dihydrofolate reductase gene in cultured mammalian cells, the role of the enhancer in immunoglobulin gene regulation, the mechanism of RNA splicing and 3' end formation, biogenesis of mitochondria in yeast, transcription termination and antitermination in E. coli, and the characterization of genes controlling cell differentiation in C. elegans. The facility would be available to all 24 laboratories of the Columbia Department of Biological Sciences, and to a limited extent, to undergraduates.