SGER: CHO cell lines and vectors for rapid development of high level recombinant protein production.

本研究的目的之一是通过确定具有高扩增率的特定染色体位置来缩短中国仓鼠卵巢（CHO）细胞的基因扩增过程。然后，这个位置将被改造以接受任何转染的基因。与此同时，一种更快速的方法将被开发出来，从而产生即时扩增。这项研究的另一个目的是创造新的质粒载体，其中任何基因都可以被引入，并指定RNA序列，赋予：1)mRNA的优化稳定性；2)翻译成蛋白质的优化能力；以及3)从细胞分泌该产物的最佳速率。这项研究将对在培养的哺乳动物细胞中制造治疗性单克隆抗体的上游加工步骤产生重大影响。在这项工作的过程中，研究生将被指导进入生物技术行业

0735385 (Chasin) One aim of this research is to shorten the gene amplification process in Chinese hamster ovary (CHO) cells by identifying a particular chromosomal location that exhibits a high rate of amplification. This location will then be engineered to accept any transfected gene. In parallel, an even more rapid method will be developed that results in instant amplification. An additional aim of this research is to create novel plasmid vectors into which any gene can be introduced, and which specify RNA sequences that confer: 1) an optimized stability of the mRNA; 2) an optimized ability to initiate translation into protein; and 3) an optimized rate of secretion of the product from the cell. The board impact of research will be on the upstream processing steps in the manufacture of therapeutic mAbs in cultured mammalian cells. In the course of this work, a graduate student will be mentored for entry into the biotechnology industry