Developmental Physiology of Migratory Neurons in the Moth Enteric Nervous System

蛾肠神经系统迁移神经元的发育生理学

• 批准号: 9010538
• 负责人: Philip Copenhaver
• 金额: $ 22.09万
• 依托单位: Oregon Health & Science University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9010538&HistoricalAwards=false
• 关键词: Developmental; Physiology; Migratory; Neurons; Moth

A central issue in developmental neurobiology concerns what factors are involved in the differentiation of ion channels in developing neurons. This issue will be studied in migrating neurons that form the enteric nervous system in the moth, Manduca. This system is uniquely suited for such studies since the developmental fates of these neurons are well characterized, there are only 300 neurons in this system and these are easily accessible for electrophysiological recording and experimental manipulation in the embryo. In addition, these neurons can be removed from the embryo and studied in culture. Intracellular and patch clamp recordings will be performed on identified neurons during and after their migration in situ and in culture to study the expression and development of voltage dependent potassium, calcium dependent potassium, sodium and calcium currents. These studies will lead in the future to molecular and genetic analyses of channel gene expression.

发育神经生物学的一个核心问题是什么因素参与了发育中的神经元离子通道的分化。这个问题将在Manduca飞蛾肠道神经系统的迁移神经元中进行研究。该系统非常适合此类研究，因为这些神经元的发育命运得到了很好的表征，该系统中只有300个神经元，这些神经元很容易在胚胎中进行电生理记录和实验操作。此外，这些神经元可以从胚胎中取出并在培养中进行研究。细胞内和膜片钳记录将对鉴定的神经元在原位和培养中迁移期间和之后进行记录，以研究电压依赖性钾、钙依赖性钾、钠和钙电流的表达和发展。这些研究将导致未来通道基因表达的分子和遗传分析。