Molecular Structure and Function Relations of Transients. Photoreceptors: Time-Resolved Infrared Studies

瞬态的分子结构和功能关系。

• 批准号: 9012617
• 负责人: Juan Lopez-Garriga
• 金额: $ 1.18万
• 依托单位: University of Puerto Rico Mayaguez
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-15 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9012617&HistoricalAwards=false
• 关键词: Molecular; Structure; Function; Relations; Transients.

This proposal seeks to provide fundamental insight into how, in the phytochrome photoreceptor (the plant growth and photoresponse control agent) and bacteriorhodopsin ( a light driven proton pump), protein structure relates to protein function and reactivity. The overall goal of this research is to study, using time-resolved infrared spectroscopy from picosecond to kilosecond time regimes, light-induced conformational changes and kinetic intermediates by following the dynamics of the protein amide I and II regions, carboxylic groups, specific amino acid (i.e. tryptophan and tyrosine) and protein photointermediates. In addition to the dynamic understanding of the interaction between light and the photosensitive bilitriene phytochrome, the investigatior will determine a) orientation and strenght of possible hydrogen bonds between the imine hydrogen and a nearby counter ion; and b) effects of a positive or negative charge near the C=N frequency shift upon protonation of both the bilitriene chromophore and the retinal Schiff's base chromophore. This proposal is also aimed at learning how the reversible photocontrol properties of phytochrome and bacteriorhodopsin can be used to produce light biosensors or photodevices.

该提案旨在提供关于光敏色素光感受器（植物生长和光反应控制剂）和细菌视紫红质（光驱动质子泵）中蛋白质结构如何与蛋白质功能和反应性相关的基本见解。本研究的总体目标是利用皮秒到千秒的时间分辨红外光谱，通过跟踪蛋白质酰胺I和II区、羧基、特定氨基酸（即色氨酸和酪氨酸）和蛋白质光中间体的动力学，研究光诱导的构象变化和动力学中间体。除了对光与光敏性bilitriene光敏色素之间相互作用的动态理解外，该研究还将确定a)亚胺氢与附近反离子之间可能的氢键的取向和强度；b) C=N频移附近的正电荷或负电荷对二烯发色团和视网膜希夫碱发色团质子化的影响。这一建议也旨在了解如何利用光敏色素和细菌视紫红质的可逆光控特性来生产光生物传感器或光器件。