Ultrapurification of Biomacromolecules

基因工程的最新进展 允许工业规模生产治疗性蛋白质 for human人的health健康care保健. 随着新的 生产方法得到了极大的净化 挑战 为了避免不良的副作用 污染物（其中许多是未知的）， 治疗性蛋白质必须被超纯化到 超出了现有技术的范围。 因此，有一个 对新的分离和纯化的巨大需求 可以在工业规模上应用的技术。 这项多研究者的研究是一项整合 几项创新技术，如高性能 液相色谱法（HPLC）、电泳和 超临界流体分离提供依据 用于生物大分子的工业超纯化。 这种新的纯化和分离的应用 技术将在重组组织上进行测试 纤溶酶原激活剂，一种用于 心脏病患者的治疗

The remarkable recent advances in genetic engineering now allow industrial-scale production of therapeutic proteins for human health care. With the introduction of new production methods have come immense purification challenges. In order to avoid untoward side effects of contaminants (many of which are as yet unknown), therapeutic proteins must be ultra-purified to levels beyond the scope of present technology. Thus, there is a great need for novel separation and purification techniques which may be applied on an industrial scale. This multi-investigator study is an effort to integrate several innovative technologies ısuch as High Performance Liquid Chromatography, (HPLC), electrophoresis, and supercritical-fluid based separations! to provide a basis for industrial ultrapurification of biomacromolecules. Applications of such new purification and separation technologies will be tested on recombinant tissue plasminogen activator, a life-saving therapeutic used in the treatment of heart attack victims.