HPLC and CZE for Protein Purification Processes

用于蛋白质纯化过程的 HPLC 和 CZE

• 批准号: 9112256
• 负责人: Csaba Horvath
• 金额: $ 5.11万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-15 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9112256&HistoricalAwards=false
• 关键词: HPLC; CZE; Protein; Purification; Processes

New HPLC and CZE equipment will provide not only a base from which the Principal Investigators (PIs) will develop new separation techniques, but also essential analytical tools to cross-check and monitor and corroborate theoretical advances. Four separation techniques will be studied: capillary zone electrophoresis, supercritical fluid-based processes, analytical chromatography, and displacement chromatography. The requested HPLC and CZE equipment will be used in these projects for analytical work and for collection of physico-chemical data required in simulation and design of the various processes. They will also be used, sometimes with modifications, to explore novel techniques to be developed for the enhancement of the efficiency of both liquid chromatography and CZE in bioprocess applications.

新的 HPLC 和 CZE 设备不仅将为首席研究员 (PI) 开发新的分离技术提供基础，而且还将提供必要的分析工具来交叉检查、监测和证实理论进展。 将研究四种分离技术：毛细管区带电泳、基于超临界流体的工艺、分析色谱法和置换色谱法。 所需的 HPLC 和 CZE 设备将在这些项目中用于分析工作以及收集各种过程的模拟和设计所需的物理化学数据。 它们还将用于探索待开发的新技术，以提高生物过程应用中液相色谱和 CZE 的效率，有时会进行修改。