U.S.-France Cooperative Research: Regulation of Ferritin Expression in Plants

该奖项将支持生物化学领域的合作研究 北卡罗来纳州的伊丽莎白·泰尔医生 Jean-Francois Briat博士， 格勒诺布尔研究的目的是调查 植物铁蛋白表达的调控。 关键反应（如细胞分裂）需要铁 和呼吸作用），以及植物特异性反应（例如， 光合作用和固氮作用）。 铁的变化 黄化病和线虫病中发生铁蛋白贮藏 感染 在动物中，铁蛋白由多基因编码， 家族，并通过铁和保守的 翻译调节序列 可比信息 缺少植物。 最近，调查人员 显示豌豆和大豆铁蛋白共有55-65%序列 鉴定为脊椎动物铁蛋白，但大豆铁蛋白 mRNA还编码一种转运肽， 铁蛋白的质体定位。 铁的作用 植物铁蛋白的合成是改变铁蛋白mRNA 浓度. 在这个结合了美国和 法国在铁蛋白和基因表达方面的专业知识， 植物，调查人员将进行联合研究， 铁期间植物中铁蛋白的调节和积累 胁迫、根瘤形成和种子发育。 铁蛋白 cDNA和抗体将用于检查：1）铁蛋白 植物基因组中的序列（调控和编码），2） 铁对铁蛋白mRNA含量和转录影响， 和3）正常期间铁蛋白mRNA和蛋白质积累 以及异常的开花、叶片成熟和种子形成。 这些结果对于了解铁的重要性 植物的铁代谢和铁蛋白调节，以及 为未来的细胞内运输提供了一个模型， 与质体发育有关。

This award will support collaborative research in biochemis- try between Dr. Elizabeth Theil, North Carolina State University, and Dr. Jean-Francois Briat, University of Grenoble. The objective of the research is to investigate the regulation of ferritin expression in plants. Iron is required for critical reactions (e.g. cell division and respiration), as well as plant-specific reactions (e.g. photosynthesis and nitrogen fixation). Alterations in iron storage in ferritin occur in chlorosis and nematode infection. In animals, ferritin is encoded in a multigene family and is regulated by iron and through a conserved translational regulatory sequence. Comparable information is lacking in plants. Recently the investigators have shown that pea and soybean ferritins share 55-65% sequence identify with vertebrate ferritins, but soybean ferritin mRNA also encodes a transit peptide apparently related to the plastid location of ferritin. The effect of iron on ferritin synthesis in plants is to change ferritin mRNA concentration. In this project, which combines US and French expertise on ferritin and on gene expression in plants, the investigators will carry out a joint study of ferritin regulation and accumulation in plants during iron stress, nodule formulation, and seed development. Ferritin cDNAs and antibodies will be used to examine: 1) ferritin sequences (regulatory and coding) in plant genomes, 2) the effect of iron on ferritin mRNA content and transcription, and 3) ferritin mRNA and protein accumulation during normal and abnormal nodulation, leaf maturation and seed formation. The results will be important for understanding iron metabolism and ferritin regulation in plants, as well as providing a model in the future for intracellular trafficking related to plastid development.