U.S.-France Cooperative Research: Regulation of Ferritin Expression in Plants

美法合作研究：植物铁蛋白表达的调控

• 批准号: 9015929
• 负责人: Elizabeth Theil
• 金额: $ 0.8万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1994-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9015929&HistoricalAwards=false
• 关键词: U.S.; France; Cooperative; Research; Regulation

This award will support collaborative research in biochemis- try between Dr. Elizabeth Theil, North Carolina State University, and Dr. Jean-Francois Briat, University of Grenoble. The objective of the research is to investigate the regulation of ferritin expression in plants. Iron is required for critical reactions (e.g. cell division and respiration), as well as plant-specific reactions (e.g. photosynthesis and nitrogen fixation). Alterations in iron storage in ferritin occur in chlorosis and nematode infection. In animals, ferritin is encoded in a multigene family and is regulated by iron and through a conserved translational regulatory sequence. Comparable information is lacking in plants. Recently the investigators have shown that pea and soybean ferritins share 55-65% sequence identify with vertebrate ferritins, but soybean ferritin mRNA also encodes a transit peptide apparently related to the plastid location of ferritin. The effect of iron on ferritin synthesis in plants is to change ferritin mRNA concentration. In this project, which combines US and French expertise on ferritin and on gene expression in plants, the investigators will carry out a joint study of ferritin regulation and accumulation in plants during iron stress, nodule formulation, and seed development. Ferritin cDNAs and antibodies will be used to examine: 1) ferritin sequences (regulatory and coding) in plant genomes, 2) the effect of iron on ferritin mRNA content and transcription, and 3) ferritin mRNA and protein accumulation during normal and abnormal nodulation, leaf maturation and seed formation. The results will be important for understanding iron metabolism and ferritin regulation in plants, as well as providing a model in the future for intracellular trafficking related to plastid development.

该奖项将支持北卡罗来纳州立大学的 Elizabeth Theil 博士和格勒诺布尔大学的 Jean-Francois Briat 博士之间的生物化学合作研究。该研究的目的是研究植物中铁蛋白表达的调控。 关键反应（例如细胞分裂和呼吸）以及植物特异性反应（例如光合作用和固氮）需要铁。 铁蛋白中铁储存的改变发生在萎黄病和线虫感染中。 在动物中，铁蛋白由多基因家族编码，并受铁和保守的翻译调节序列的调节。 植物中缺乏类似的信息。 最近，研究人员表明，豌豆和大豆铁蛋白与脊椎动物铁蛋白有 55-65% 的序列同一性，但大豆铁蛋白 mRNA 还编码显然与铁蛋白质体位置相关的转运肽。 铁对植物铁蛋白合成的影响是改变铁蛋白mRNA浓度。 在这个项目中，结合了美国和法国在铁蛋白和植物基因表达方面的专业知识，研究人员将联合研究铁胁迫、根瘤形成和种子发育过程中植物中铁蛋白的调节和积累。 铁蛋白 cDNA 和抗体将用于检查：1) 植物基因组中的铁蛋白序列（调控和编码），2) 铁对铁蛋白 mRNA 含量和转录的影响，以及 3) 正常和异常结瘤、叶子成熟和种子形成过程中铁蛋白 mRNA 和蛋白质的积累。 这些结果对于了解植物中的铁代谢和铁蛋白调节非常重要，并为未来与质体发育相关的细胞内运输提供模型。