Interactions of Specific Ligands with Proteins from Peanut, Sunflower Seed, Safflower Seed and Sesame Seed, Award in U.S and Indian Currencies

描述：该项目支持马萨诸塞州沃尔瑟姆市布兰代斯大学的Serge Timasheff博士和印度迈索尔中央食品技术研究所的V. Prakash博士的合作研究。这项研究旨在了解油籽（如向日葵、芝麻、花生和棉花籽）中蛋白质和各种配体之间的相互作用。这些配体的存在阻止了这些种子中蛋白质的充分利用。本研究将完成高分子量蛋白和低分子量蛋白的分离以及构象配体的分离。研究单个蛋白质和单个配体对的相互作用，确定不同反应条件下的结果，并了解结合位点的化学计量学、几何和反应动力学的性质。本研究将使用多种生物物理技术，包括光谱偏振法、分析超离心法、荧光偏振法、亲和色谱法和分光光度法。范围：这项研究汇集了一位知名的美国科学家和一位来自印度的高产青年研究人员，共同研究生物物理学和生物化学领域的基础科学问题。此外，这项研究在提高许多欠发达国家满足其营养需求的能力方面具有潜在的重大益处。两位科学家将相互补充，以提高预期的研究成果。这个项目符合美印合作科学计划的目标。资助：本项目获以下两项拨款资助：布兰代斯大学食品研究中心__________________ _____________________财政美元印度货币___________________ _________________ 909,001 2,94123,529 91 6,231 2941 42,947 92 6,230 2941 25,259 93 ----- ----- 24,671

Description: This project supports collaborative research by Dr. Serge Timasheff of Brandeis University, Waltham, MA, and Dr. V. Prakash of the Central Food Technology Research Institute in Mysore, India. The research is aimed at understanding the interaction between proteins and various ligands present in oil seeds such as sunflower, sesame, peanut, and cotton seeds. The presence of these ligands prevents the full utilization of the proteins in these seeds. In this research, isolation of high and low molecular weight proteins as well as isolation of constituant ligands will be completed. A study will be made of the interactions of pairs of individual proteins and individual ligands, to determine the results under different reaction conditions, and to understand the nature of the binding site stoichiometry, geometry and reaction kinetics. Various biophysical techniques will be used in the study including spectropolarimetry, analytical ultracentrifugation, fluoresence polarisation, affinity chromatography, and spectrophotometry. Scope: This research brings together a well-established U.S. scientist and a highly productive young researcher from India to work on a problem of basic scientific interest in the biophysics and biochemistry areas. In addition, this research has potentially significant benefits in improving the capabilities of many Less Developed Countries to meet their nutritional needs. The two scientists will complement each others' work to enhance the expected research output. This project meets the objectives of the U.S.-India Cooperative Science Program. Funding: This project is being funded under two grants as follows: Brandeis Univ. Centr Food Res. Inst. __________________ _____________________ FY U.S. $ Indian Curr $ Indian Currency $ ______ _____________ _________________ 90 9,001 2,941 23,529 91 6,231 2,941 42,947 92 6,230 2,941 25,259 93 ----- ----- 24,671