Interaction of Specific Ligands with Proteins from Peanut, Sunflower Seed, Safflower Seed and Sesame Seed, Award in Indian Rupees

描述：该项目支持马萨诸塞州沃尔瑟姆布兰迪斯大学的Serge Timasheff博士和印度迈索尔中央食品技术研究所的V.Prakash博士的合作研究。这项研究旨在了解葵花籽、芝麻、花生和棉花种子中存在的蛋白质和各种配体之间的相互作用。这些配体的存在阻碍了这些种子中蛋白质的充分利用。在这项研究中，将完成高、低分子量蛋白质的分离以及组成配体的分离。将对单个蛋白质和单个配体的相互作用进行研究，以确定在不同反应条件下的结果，并了解结合位置的性质、化学计量学、几何和反应动力学。研究中将使用各种生物物理技术，包括光谱偏振、分析超速离心法、荧光偏振、亲和层析和分光光度法。范围：这项研究汇集了一位久负盛名的美国科学家和一位来自印度的高生产率的年轻研究人员，致力于解决生物物理和生物化学领域的基本科学兴趣问题。此外，这项研究在改善许多欠发达国家满足其营养需求的能力方面具有潜在的重大好处。这两位科学家将相互补充工作，以提高预期的研究产出。该项目符合美印合作科学计划的目标。资金：该项目由以下两项赠款提供资金：布兰迪斯大学。食品研究中心Inst.__90 9,001 2,941 23,529 91 6,231 2,941 42,947 92 6,230 2,941 25,259 93-24,671

Description: This project supports collaborative research by Dr. Serge Timasheff of Brandeis University, Waltham, MA, and Dr. V. Prakash of the Central Food Technology Research Institute in Mysore, India. The research is aimed at understanding the interaction between proteins and various ligands present in oil seeds such as sunflower, sesame, peanut, and cotton seeds. The presence of these ligands prevents the full utilization of the proteins in these seeds. In this research, isolation of high and low molecular weight proteins as well as isolation of constituant ligands will be completed. A study will be made of the interactions of pairs of individual proteins and individual ligands, to determine the results under different reaction conditions, and to understand the nature of the binding site stoichiometry, geometry and reaction kinetics. Various biophysical techniques will be used in the study including spectropolarimetry, analytical ultracentrifugation, fluoresence polarisation, affinity chromatography, and spectrophotometry. Scope: This research brings together a well-established U.S. scientist and a highly productive young researcher from India to work on a problem of basic scientific interest in the biophysics and biochemistry areas. In addition, this research has potentially significant benefits in improving the capabilities of many Less Developed Countries to meet their nutritional needs. The two scientists will complement each others' work to enhance the expected research output. This project meets the objectives of the U.S.-India Cooperative Science Program. Funding: This project is being funded under two grants as follows: Brandeis Univ. Centr Food Res. Inst. __________________ _____________________ FY U.S. $ Indian Curr $ Indian Currency $ ______ _____________ _________________ 90 9,001 2,941 23,529 91 6,231 2,941 42,947 92 6,230 2,941 25,259 93 ----- ----- 24,671