Interaction of Specific Ligands with Proteins from Peanut, Sunflower Seed, Safflower Seed and Sesame Seed, Award in Indian Rupees

特定配体与花生、葵花籽、红花籽和芝麻籽蛋白质的相互作用，印度卢比奖

• 批准号: 9016271
• 负责人: V Prakash
• 金额: $ 9.79万
• 依托单位: Central Food Technological Research Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-15 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9016271&HistoricalAwards=false
• 关键词: Interaction; Specific; Ligands; Proteins; Peanut

Description: This project supports collaborative research by Dr. Serge Timasheff of Brandeis University, Waltham, MA, and Dr. V. Prakash of the Central Food Technology Research Institute in Mysore, India. The research is aimed at understanding the interaction between proteins and various ligands present in oil seeds such as sunflower, sesame, peanut, and cotton seeds. The presence of these ligands prevents the full utilization of the proteins in these seeds. In this research, isolation of high and low molecular weight proteins as well as isolation of constituant ligands will be completed. A study will be made of the interactions of pairs of individual proteins and individual ligands, to determine the results under different reaction conditions, and to understand the nature of the binding site stoichiometry, geometry and reaction kinetics. Various biophysical techniques will be used in the study including spectropolarimetry, analytical ultracentrifugation, fluoresence polarisation, affinity chromatography, and spectrophotometry. Scope: This research brings together a well-established U.S. scientist and a highly productive young researcher from India to work on a problem of basic scientific interest in the biophysics and biochemistry areas. In addition, this research has potentially significant benefits in improving the capabilities of many Less Developed Countries to meet their nutritional needs. The two scientists will complement each others' work to enhance the expected research output. This project meets the objectives of the U.S.-India Cooperative Science Program. Funding: This project is being funded under two grants as follows: Brandeis Univ. Centr Food Res. Inst. __________________ _____________________ FY U.S. $ Indian Curr $ Indian Currency $ ______ _____________ _________________ 90 9,001 2,941 23,529 91 6,231 2,941 42,947 92 6,230 2,941 25,259 93 ----- ----- 24,671

描述：本项目支持合作研究 由马萨诸塞州沃尔瑟姆市布兰代斯大学的Serge Timasheff博士， 和中央食品技术研究所的V.普拉卡什博士 印度迈索尔的研究所。 该研究旨在 了解蛋白质和各种 存在于油料种子如向日葵，芝麻， 花生和棉花种子。 这些配体的存在 阻止了这些蛋白质的充分利用， 种子 在这项研究中，高和低的隔离 分子量蛋白质以及分离 将完成组成配体。 将进行研究 蛋白质对之间的相互作用， 单个配体，以确定不同条件下的结果 反应条件，并了解的性质， 结合位点化学计量、几何形状和反应动力学。 研究中将使用各种生物物理技术 包括分光偏振法、分析 超离心，荧光偏振，亲和性 色谱法和分光光度法。 范围：这项研究汇集了一个完善的 美国科学家和一位高产的年轻研究人员， 印度将致力于解决一个基本的科学问题， 生物物理学和生物化学领域。 另外这款 研究有潜在的重大利益， 许多最不发达国家的能力，以满足 他们的营养需求。 两位科学家将补充 彼此的工作，以提高预期的研究成果。 该项目符合美国的目标-印度 合作科学计划。 资金：该项目由两项赠款资助， 如下所示： 布兰迪斯大学 中央食品研究所 __________________ _____________________ FY 美元印度Curr $ 印度货币$ ______ _____________ _________________ 90 9,001 2,941 23,529 91 6,231 2,941 42,947 92 6,230 2,941 25,259 93 ----- ----- 24,671