Pattern Formation During Oogenesis in D. melanogaster

本提案的长期目标是了解如何 在果蝇卵中建立了位置信息 在卵子发生过程中。科恩博士专注于表达， K10和C因子基因。他对K10的兴趣源于 从其已知的建立背腹 卵的极性; K10基因活性缺乏的雌性 产生畸形的卵子，如果受精， 胚胎科恩博士最近的数据表明，K10是转录 只存在于滋养细胞中，但它的转录本 选择性地快速运输到卵母细胞中， 被翻译了。遗传和分子方法将用于 鉴定参与K10 RNA转运的蛋白质， 将细胞培育成卵母细胞。科恩博士对C因子干细胞的兴趣 从它作为转录激活因子的潜在作用， 滋养细胞和卵母细胞中的转录阻遏物。的 营养细胞中RNA的专一性合成似乎是一种 这是随后将一些RNA定位到 卵母细胞的特定区域。为了阐明C因子的功能， 目的：1）确定C因子蛋白在护士中的分布 细胞和卵母细胞，和2）鉴定/产生C因子突变体。

The long term goal of this proposal is to understand how positional information is established in the Drosophila egg during oogenesis. Dr. Cohen is focussing on the expression and function of the K10 and C factor genes. His interest in K10 stems from its known requirement in the establishment of dorso-ventral polarity in the egg; females deficient for K10 gene activity produce abnormally shaped eggs that, if fertilized, die as embryos. Dr. Cohen's recent data indicate that K10 is transcribed exclusively in nurse cells, but that its transcripts are selectively and rapidly transported into the oocyte where they are translated. Genetic and molecular approaches will be used to identify the proteins involved in the transport of K10 RNA from nurse cells to oocytes. Dr. Cohen's interest in C factor stems from its potential role as both an activator of transcription in nurse cells and a repressor of transcription in oocytes. The exclusive synthesis of RNAs in nurse cells appears to be a prerequisite for the subsequent localization of some RNAs to specific regions of the oocyte. To elucidate C factor function he will; 1) determine the distribution of C factor protein in nurse cells and oocytes, and 2) identify/generate C factor mutants.