G-Proteins in the Response System Phycomyces blakesleeanus

低等真菌黑藻（Phycomyces blakesleeanus）的单细胞孢子囊对蓝光非常敏感，在光照下向光弯曲生长，对光照的减少表现出负的生长响应。藻菌在比较低等生物和高等生物的信号处理方面具有很大的潜力。该研究人员已经发现了G蛋白存在于孢子囊中的证据，G蛋白在高等生物的光导和许多类型的信号转导中是必不可少的。在本提案中，研究者将寻求建立G蛋白在藻属蓝光转导中的作用。用G（亚基）抗体和共价FAD（与真菌的感光系统有关）的免疫印迹法将用于许多行为突变菌株。cDNA文库将用于克隆G（亚基），并将其与其他系统中的这些蛋白质进行比较，来自突变体的mrna将通过Northern blotting进行检测。这些研究将提供蓝光检测系统如何在分子水平上工作的重要信息，并将指出低等真核生物和脊椎动物的信号转导系统之间存在的共同要素。＊＊＊

Abstract 9514026 Cohen The single-cell sporangiophore of the lower fungus Phycomyces blakesleeanus is very sensitive to blue light, by bending toward the light and growing with exposure to light, and showing a negative growth response to a decrease in light. Phycomyces has great potential for comparing signal processing in lower organisms to those in higher organisms. This investigator has found evidence for the presence of G proteins in the sporangiophore, and G proteins are essential for phototransduction and many types of signal transduction in higher organisms. In this proposal the investigator will seek to establish the role of G proteins in blue light transduction in Phycomyces. Immunoblotting with antibodies to G( subunits and covalent FAD (which is involved in the photoreceptor system of this fungus), will be used in a number of behavioral mutant strains. A cDNA library will be used for cloning of G( subunits and these will be compared to these proteins in other systems, and mRNAs from the mutants will be examined by Northern blotting. These studies will provide important information on how the blue light detection system works at the molecular level and will indicate common elements that exists between signal transduction systems of the lower eukaryotic organisms and vertebrates. ***