T-DNA Tagged Photosynthetic Mutant Genes in Arabidopsis

拟南芥中 T-DNA 标记的光合突变基因

• 批准号: 9018318
• 负责人: Alice Cheung
• 金额: $ 27万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018318&HistoricalAwards=false
• 关键词: DNA; Tagged; Photosynthetic; Mutant; Genes

The research proposed will use T-DNA tagged mutants in Arabidopsis to identify and isolate genes related to photosynthesis in this model plant, and seeks to determine the roles of these genes in photosynthetic development and other functions. The tagged genes of two albino mutants and their corresponding wild-type alleles will be examined in the effort to reach the goals stated. The effects of the tagged mutations on the molecular phenotype of mutant plants will be examined, and the properties of encoded proteins assessed in the effort to reach mechanistic explanations for association of phenotype and mutant. Many mutations in the photosynthetic process lead to pleiotropic molecular phenotypes and this makes difficult deriving a mechanistic explanation of the relationship between a phenotype and a mutant. Use of T-DNA tagged genes in mutants and wild types should permit a precise correlation between isolated genes and their functions in photosynthesis.

这项研究将使用拟南芥中的T-DNA标记突变体 在这一过程中识别和分离与光合作用相关的基因， 模式植物，并试图确定这些基因的作用， 光合作用和其他功能。 标记的基因 两个白化病突变体及其相应的野生型等位基因 将在努力实现所述目标的过程中加以审查。 的 标记突变对人乳腺癌细胞分子表型的影响 突变植物将被检查，和编码的属性， 为了达到机械解释而评估的蛋白质 表型和突变体的关联。 光合作用过程中的许多突变导致多效性 分子表型，这使得很难推导出 对表型与 一个变种人 T-DNA标记的基因在突变体和野生型中的应用 应该允许分离的基因之间的精确关联， 它们在光合作用中的功能。