Molecular Evaluation of Disease Resistance and Susceptibility in Oats

燕麦抗病性和易感性的分子评价

• 批准号: 9018571
• 负责人: Thomas Wolpert
• 金额: $ 15万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018571&HistoricalAwards=false
• 关键词: Molecular; Evaluation; Disease; Resistance; Susceptibility

This proposal is directed at evaluating whether a victorin-binding protein (VBP) isolated from oat is the receptor for this toxin and is the molecular determinant of susceptibility to the disease Victoria bight. Using purified VBP, antibody thereto, and a full length cDNA already cloned, the functional role of VBP will be examined in biochemical aspects, the binding domains examined, sequence of VBP-cDNA determined, a determination of segregation of the gene encoding VBP studied, and sequence heterogeneity in VBP of resistant and susceptible phenotypes compared. This work is directed at a critical issue in modern plant pathology, namely an understanding of the site and function of a plant gene product which regulated the pathogenicity of a fungal pathogen that causes the disease, Victoria bight, of oat.

该建议旨在评估是否有一种具有Victorin约束力的 从燕麦中分离的蛋白质（VBP）是这种毒素的受体， 是疾病易感性的分子决定因素 维多利亚湾。 使用纯化的VBP、其抗体和完整的 长度cDNA已经克隆，VBP的功能作用将是 在生物化学方面进行了检查，检查了结合结构域， 测定VBP-cDNA的序列， 研究了编码VBP的基因，以及VBP的序列异质性， 抗性和敏感表型的比较。 这项工作是针对一个关键问题，在现代工厂 病理学，即了解的网站和功能的一个 调节真菌致病性的植物基因产物 引起燕麦维多利亚病的病原体。