Molecular Evaluation of Disease Resistance and Susceptibility in Oats

这项建议旨在评估从燕麦中分离出来的Victorin结合蛋白(VBP)是否是这种毒素的受体，以及是否是维多利亚白斑病易感性的分子决定因素。利用纯化的VBP及其抗体和已克隆的全长cDNA，将从生化方面检测VBP的功能作用，检查结合区域，确定VBP-cDNA的序列，确定编码VBP的基因的分离，并比较VBP中抗性和感病表型的序列异质性。这项工作是针对现代植物病理学中的一个关键问题，即了解植物基因产物的位置和功能，该基因产物调节引起燕麦维多利亚褐斑病的真菌病原菌的致病性。

This proposal is directed at evaluating whether a victorin-binding protein (VBP) isolated from oat is the receptor for this toxin and is the molecular determinant of susceptibility to the disease Victoria bight. Using purified VBP, antibody thereto, and a full length cDNA already cloned, the functional role of VBP will be examined in biochemical aspects, the binding domains examined, sequence of VBP-cDNA determined, a determination of segregation of the gene encoding VBP studied, and sequence heterogeneity in VBP of resistant and susceptible phenotypes compared. This work is directed at a critical issue in modern plant pathology, namely an understanding of the site and function of a plant gene product which regulated the pathogenicity of a fungal pathogen that causes the disease, Victoria bight, of oat.