A Downside to Disease Resistance in Arabidopsis: The Trouble with LOV

拟南芥抗病性的一个缺点：LOV 的麻烦

• 批准号: 0724954
• 负责人: Thomas Wolpert
• 金额: $ 53.58万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0724954&HistoricalAwards=false
• 关键词: Downside; Disease; Resistance; Arabidopsis; Trouble

The overall objective of this research is to understand the physiological basis of plant disease susceptibility. To accomplish this, a disease in the model plant, Arabidopsis thaliana is being investigated because of its genetic simplicity. The disease is conditioned by a single dominant host gene and is caused by the fungus Cochliobolus victoriae whose virulence depends on the production of a "toxin" called victorin. If a victorin-producing isolate encounters a plant carrying the gene for susceptibility, disease occurs. In all other cases, such as a lack of victorin production, or a plant lacking the susceptibility gene, disease does not occur. Because susceptibility is completely dependent on a single gene, understanding the function of that gene should contribute to understanding plant disease susceptibility. In Arabidopsis, susceptibility is conferred by a gene called LOV (for locus orchestrating victorin sensitivity) which encodes a CC-NBS-LRR type protein. Ironically, the only known function for this type of protein is in conferring disease resistance. Nonetheless, LOV conditions disease susceptibility. The identification of LOV presents a fundamentally different perspective on plant resistance gene function and implies that the induction of resistance can entail physiological changes that actually increase a plant's vulnerability to pathogens. The work to be conducted will investigate this possibility through a combination of approaches that will determine whether known resistance responses can lead to disease susceptibility and conversely, whether the response conferred by LOV can confer disease resistance. We anticipate that both outcomes are likely. The broader impacts of this effort will be integrated into both undergraduate and graduate level education. For example, the plant response will be used as the basis for a laboratory exercise in an undergraduate course. Also, the research will provide experiences for graduate and postdoctoral researchers whose results will be disseminated through publications and seminars.

本研究的总体目标是了解植物病害易感性的生理基础。 为了实现这一目标，正在研究模式植物拟南芥中的一种疾病，因为它的遗传简单性。 这种疾病是由一个单一的显性宿主基因，并由真菌Cochliobolus victoriae引起的，其毒性取决于一种名为Victorin的“毒素”的产生。 如果一个产生胜利素的隔离物遇到一个携带易感基因的植物，疾病就会发生。 在所有其他情况下，如缺乏Victorin生产，或缺乏易感基因的植物，疾病不会发生。由于易感性完全依赖于单个基因，了解该基因的功能应有助于了解植物疾病的易感性。在拟南芥中，易感性由称为LOV（用于协调维克托林敏感性的基因座）的基因赋予，该基因编码CC-NBS-LRR型蛋白。具有讽刺意味的是，这种蛋白质的唯一已知功能是赋予抗病性。尽管如此，LOV条件疾病的易感性。 LOV的鉴定对植物抗性基因功能提出了一个根本不同的观点，并意味着抗性的诱导可能引起实际上增加植物对病原体的脆弱性的生理变化。即将开展的工作将通过一系列方法来研究这种可能性，这些方法将确定已知的抗性反应是否会导致疾病易感性，反之，LOV赋予的反应是否会赋予疾病抗性。 我们预计这两种结果都有可能。这一努力的更广泛影响将被纳入本科和研究生教育。例如，植物的反应将被用作本科课程实验室练习的基础。此外，研究将为研究生和博士后研究人员提供经验，其结果将通过出版物和研讨会传播。