Molecular Genetics of Fertilization in Arabidopsis

拟南芥受精的分子遗传学

• 批准号: 9018889
• 负责人: Robert Pruitt
• 金额: $ 36万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1992-09-25
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018889&HistoricalAwards=false
• 关键词: Molecular; Genetics; Fertilization; Arabidopsis

Cell-cell recognition is a fundamental biological process which underlies many critical developmental events. An example of a process which relies heavily on cell-cell interactions is fertilization in higher plants. The research described in this proposal is designed to contribute to our understanding of the mechanisms which are involved in these cell-cell interactions, as well as to increase our knowledge of how these interactions act to regulate the fertilization process. A genetic approach will be used to identify mutations in Arabidopsis thaliana which disrupt the process of fertilization and these mutations will be characterized to determine which are involved in cell-cell interactions. The genes identified by the mutations will be cloned using a combination of RFLP mapping and "chromosome walking". The cloned genes will then be used to characterized the expression patterns and products of the genes. In a parallel project, a small family of Arabidopsis genes showing sequence similarity to those involved in self-recognition during fertilization in Brassica oleracea will be characterized to determine if any members of the family play a role in other recognition events during fertilization. By identifying and characterizing gene products which are part of the interaction process we will learn about the molecular mechanisms of these interactions.

细胞-细胞识别是一个基本的生物学过程， 是许多重要的发展事件的基础。的示例 这一过程严重依赖于细胞间的相互作用， 高等植物的受精作用本文中描述的研究 建议旨在促进我们对 参与这些细胞间相互作用的机制，如 以及增加我们对这些相互作用的了解 来调节受精过程遗传学方法将是 用于鉴定拟南芥中的突变， 受精的过程和这些突变 其特征在于确定哪些参与细胞-细胞 交互.通过突变识别的基因将是 使用RFLP作图和“染色体”相结合的方法克隆 行走”。克隆的基因将被用来表征 基因的表达模式和产物。以平行 项目，一个小家族的拟南芥基因显示序列 类似于那些参与自我认知的人， 甘蓝施肥的特点是， 确定家庭成员是否在其他方面发挥作用 受精过程中的识别事件。通过识别和 表征作为相互作用的一部分的基因产物 在这个过程中，我们将了解这些分子机制， 交互.