Characterization of dekl: A Maize Embryo-Lethal Mutation

该项目的目标是获得更好的理解 通过分析dek 1基因在植物胚胎发生中的作用 基因座谢里丹博士将在第1天描述10种突变 基因座所有十个突变等位基因产生一个隐性胚胎致死 和缺乏花色素苷的缺陷胚乳表型， 类胡萝卜素dek 1突变体胚在原胚处受阻 不能形成茎顶端分生组织，但可以 在发育后期扩大并形成根顶端分生组织。的 分析突变等位基因的表型一致性， 通过剂量分析的表达水平，包括缺失 问题研究其中五个等位基因出现在活跃的种群中， 转座因子Mutator和其中两个等位基因显示 易变性对这些等位基因之一进行分离分析， Southern印迹和与Mu 1探针杂交显示， 共分离DNA条带。该等位基因将用于初始 克隆工作，同时继续寻找新的标记等位基因 和DNA共分离带。克隆的突变等位基因将是 用于鉴定正常等位基因克隆并分离cDNA 分身后者将用于研究空间和时间 在发育中的胚胎中表达。X射线感应扇区将 用于检查dek 1形态发生的细胞自主性 表达，并评估该基因座在花青素中的作用， 基因表达。将诱导新的1 S B-A易位， 有助于基因和结构分析除了 研究了抑制茎尖分生组织形成的 dek 1突变，X射线和结构方法将用于 跟进有希望的初步观察， 正常胚茎尖分生组织的形成和 受辐射的正常胚胎调节和产生 多个胚胎

The goal of this project is to obtain a greater understanding of the role of genes in plant embryogenesis by analyzing the dek1 locus. Dr. Sheridan will characterize ten mutations at the dek1 locus. All ten mutant alleles produce a recessive embryo lethal and a defective endosperm phenotype lacking anthocyanin and carotinoids. The dek1 mutant embryo is blocked at the proembryo state and is unable to form a shoot apical meristem but can enlarge and form a root apical meristem late in development. The mutant alleles will be analyzed for uniformity of phenotype and level of expression by dosage analysis including deletion studies. Five of the alleles arose in stocks that were active for the transposable element Mutator and two of these alleles display mutability. Segregation analysis on one of these alleles by Southern blotting and hybridizing with a Mu1 probe has revealed a cosegregating DNA band. This allele will be used for an initial cloning effort while continuing to search for new tagged alleles and DNA cosegregating bands. The cloned mutant allele will be used to identify a normal allele clone and to isolate a cDNA clone. The latter will be used to examine spatial and temporal expression in developing embryos. X-ray induced sectors will be used to examine the cellular autonomy of dek1 morphogenesis expression, and to evaluate the role of this locus in anthocyanin gene expression. New 1S B-A translocation will be induced that will aid in the genetic and structural analysis. In addition to studying the blockage of shoot apical meristem formation by the dek1 mutation, X-ray and structural approaches will be used to follow up promising preliminary observation of x-ray blockage of shoot apical meristem formation of normal embryos and the capacity of irradiated normal embryos to regulate and produce multiple embryos.