Nitrogen Use Efficiency As A Strategy For Drought AdaptationIn Beans

本规划补助金提案旨在评估 非破坏性取样的准确度和精确度 这项技术将使研究人员能够获得有关 通过周期性切除的N分配和再活化 来自单个植物的相同叶子的叶盘。 这可以大大减少时间课程的成本 在空间和劳动力方面，15N次实验， 协助获取信息， 评估氮在植物策略中的作用， 提高生产力和抗压力能力 环境. 该方法将促进研究 在这个领域，可以同时使用14C， 使研究C和N的分配和再活化， 不同的环境、基因型和物种。 的 这些技术将可在实地使用， 环境，并可能被证明是一个有价值的筛选 如果氮分配和 重新动员被认为是一种策略， 提供对水分压力的适应，或者普遍地 或在经历特定类型的 干旱

This planning grant proposal is designed to evaluate the accuracy and precision of a non-destructive sampling technique that would enable researchers to acquire data on N partitioning and remobilization by periodically excising leaf discs from the same leaves of individual plants. This could substantially reduce the cost of time-course 15N experiments in terms of space and labor, while greatly facilitating the acquisition of information to help assess the role of N in plant strategies which result in increased productivity and resistance to stressful environments. The proposed method would promote research in this area and could be used simultaneously with 14C to enable study of C and N partitioning and remobilization in diverse environments, genotypes and species. The technique would be usable in field and controlled environments and could prove to be a valuable screening tool useful with plant breeding if N partitioning and remobilization are found to be one of the strategies which provide adaptation to moisture stress either universally or in environments that experience a specific type of drought.