H+-ATPase Subunits as Phylogenetic Markers

H-ATP酶亚基作为系统发育标记

• 批准号: 9020868
• 负责人: Johann Peter Gogarten
• 金额: $ 13.5万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-15 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9020868&HistoricalAwards=false
• 关键词: H+; ATPase; Subunits; Phylogenetic; Markers

Transport ATPases play a central role in the bioenergetics of all living cells. In the past we have shown that the F-(eubacterial) and V-type (eukaryotic and archaebacterial) ATPases are very useful molecular markers for early organismal evolution. The proposed research aims to extend the use of the nuclear encoded ATPAse subunits to the early radiation of eukaryotes (especially flagellates and algae) and to the evolution of land plants. Parts of the genes encoding the catalytic subunits of V-type ATPASes will be amplified directly from the genomic or cDNA of various organisms using the polymerase chain reaction (PCR). The obtained sequences will be used to determine the phylogenetic relations between these organisms, including evaluations of the significance of the derived branching patterns.

转运三磷酸腺苷酶在所有活细胞的生物能量学中起着核心作用。在过去，我们已经证明F-型（真核生物）和v型（真核生物和古细菌）atp酶是早期生物进化中非常有用的分子标记。提出的研究旨在将核编码atp酶亚基的使用扩展到真核生物（特别是鞭毛虫和藻类）的早期辐射和陆地植物的进化中。编码v型atp酶催化亚基的部分基因将使用聚合酶链反应（PCR）直接从各种生物体的基因组或cDNA中扩增出来。获得的序列将用于确定这些生物之间的系统发育关系，包括评估衍生分支模式的意义。