Nitro-Aromatic Biodegradation: Pathway Analysis and Augmentation via Genetic Engineering

硝基芳香族生物降解：通过基因工程进行途径分析和增强

• 批准号: 9023421
• 负责人: Steven Peretti
• 金额: $ 9.07万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9023421&HistoricalAwards=false
• 关键词: Nitro; Aromatic; Biodegradation; Pathway; Analysis

This proposal will extend on-going experimental work on the degradation of p-nitrophenolic wastes by an enriched Pseudomonas strain. A unique membrane reactor is to be developed that immobilizes the cells and provides for multi-phase processing. Such a reactor can isolate the active biomass from a waste stream while permitting transport of the toxicant to the biomass, and allows the maintenance of optimal culture conditions for maximum degradative activity. The effects of immobilization on cell physiology and gene expression will be addressed as a function of system operating conditions.

这项建议将扩大正在进行的利用强化假单胞菌菌株降解对硝基苯酚废物的实验工作。将开发一种独特的膜反应器，使细胞固定化，并提供多阶段处理。这样的反应器可以将活性生物质从废流中分离出来，同时允许将毒物输送到生物质中，并允许保持最佳的培养条件，以最大限度地发挥降解活性。固定化对细胞生理和基因表达的影响将作为系统运行条件的函数进行讨论。