Nitro-Aromatic Biodegradation: Pathway Analysis and Augmentation via Genetic Engineering

这一建议将扩展正在进行的利用富集假单胞菌菌株降解对硝基酚废物的实验工作。将开发一种独特的膜反应器，用于固定细胞并提供多相处理。这样的反应器可以从废物流中分离活性生物质，同时允许将毒物输送到生物质中，并允许维持最佳培养条件以获得最大的降解活性。固定化对细胞生理和基因表达的影响将作为系统操作条件的功能来解决。

This proposal will extend on-going experimental work on the degradation of p-nitrophenolic wastes by an enriched Pseudomonas strain. A unique membrane reactor is to be developed that immobilizes the cells and provides for multi-phase processing. Such a reactor can isolate the active biomass from a waste stream while permitting transport of the toxicant to the biomass, and allows the maintenance of optimal culture conditions for maximum degradative activity. The effects of immobilization on cell physiology and gene expression will be addressed as a function of system operating conditions.