Research Initiation: Protein Elicitation of Secondary Metabolites in Cultured Plant Cells

研究启动奖：植物细胞次生代谢产物的蛋白质激发本建议试图：-通过在细胞内放置一个公认的启动子蛋白并监测细胞的反应来确定启动子活性的机制，从而绕过假设的多糖外壁受体位点。评估酪氨酸脱羧酶（TDC）水平与次级代谢物小檗碱合成相关的观察结果。所得到的数据将用于建立反映对激发子浓度依赖性的生长速率、产物合成和分泌速率的动力学模型。直到更多关于次生代谢物产生的代谢控制的细节被了解，有效地利用植物细胞培养将是非常困难的。

RESEARCH INITIATION AWARD: PROTEIN ELICITATION OF SECONDARY METABOLITES IN CULTURED PLANT CELLS ABSTRACT This proposal attempts to: - determine the mechanism of elicitor activity by placing a recognized elicitor protein inside a cell and monitoring the cell's response, thereby bypassing the hypothesized polysaccharide receptor sites on the outer wall, and - evaluate the observation that tyrosine decarboxylase (TDC) levels correlate with synthesis of a secondary metabolite, berberine. The resulting data are to be used to develop kinetic models for growth rate, product synthesis and secretion rates which reflect the dependence on elicitor concentration. Until more detail is known regarding the metabolic control involved in secondary metabolite production, effective use of plant cell culture will be extremely difficult to achieve.