Research Initiation: Protein Elicitation of Secondary Metabolites in Cultured Plant Cells

研究启动奖：培养植物细胞中次生代谢物的蛋白质诱导摘要本提案试图：-通过将公认的激发子蛋白放置在细胞内并监测细胞的反应来确定激发子活性的机制，从而绕过外壁上假想的多糖受体位置，并评估酪氨酸脱羧酶(TDC)水平与次生代谢物黄连素的合成相关的观察结果。得到的数据将被用来建立生长速度、产物合成和分泌速度的动力学模型，这些模型反映了激发子浓度的依赖关系。在知道更多关于次生代谢物产生的代谢控制的细节之前，有效利用植物细胞培养将是极其困难的。

RESEARCH INITIATION AWARD: PROTEIN ELICITATION OF SECONDARY METABOLITES IN CULTURED PLANT CELLS ABSTRACT This proposal attempts to: - determine the mechanism of elicitor activity by placing a recognized elicitor protein inside a cell and monitoring the cell's response, thereby bypassing the hypothesized polysaccharide receptor sites on the outer wall, and - evaluate the observation that tyrosine decarboxylase (TDC) levels correlate with synthesis of a secondary metabolite, berberine. The resulting data are to be used to develop kinetic models for growth rate, product synthesis and secretion rates which reflect the dependence on elicitor concentration. Until more detail is known regarding the metabolic control involved in secondary metabolite production, effective use of plant cell culture will be extremely difficult to achieve.