Research Initiation: Protein Elicitation of Secondary Metabolites in Cultured Plant Cells

研究启动奖： 蛋白质诱导的次级代谢产物 培养的植物细胞 摘要 该提案试图： - 确定激发子活动的机制， 识别细胞内的激发子蛋白， 细胞的反应，从而绕过假设的 外壁上的多糖受体位点，和 - 评价酪氨酸脱羧酶（TDC） 水平与次级代谢物的合成相关， 黄连素 由此产生的数据将用于开发动力学模型， 生长率、产品合成率和分泌率， 对激发子浓度的依赖性。 直到更多的细节 已知的关于参与次级代谢控制 代谢产物的生产，有效地利用植物细胞培养将 非常难以实现。

RESEARCH INITIATION AWARD: PROTEIN ELICITATION OF SECONDARY METABOLITES IN CULTURED PLANT CELLS ABSTRACT This proposal attempts to: - determine the mechanism of elicitor activity by placing a recognized elicitor protein inside a cell and monitoring the cell's response, thereby bypassing the hypothesized polysaccharide receptor sites on the outer wall, and - evaluate the observation that tyrosine decarboxylase (TDC) levels correlate with synthesis of a secondary metabolite, berberine. The resulting data are to be used to develop kinetic models for growth rate, product synthesis and secretion rates which reflect the dependence on elicitor concentration. Until more detail is known regarding the metabolic control involved in secondary metabolite production, effective use of plant cell culture will be extremely difficult to achieve.