Instrumentation for Molecular Genetics

分子遗传学仪器

• 批准号: 9050490
• 负责人: George Kantor
• 金额: $ 2.3万
• 依托单位: Wright State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-15 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9050490&HistoricalAwards=false
• 关键词: Instrumentation; Molecular; Genetics

The lack of laboratory experiences in molecular genetics is being remedied by developing a laboratory course in molecular biology that is taken concurrently with the Molecular Biology lecture component. Laboratory experiences include DNA extractions and plasmic isolations, DNA detection and quantitation methods, restriction enzyme digestions, ligations of restriction fragments, gene marker selection procedures, agarose gel electrophoresis, and DNA transfer and hybridization methods. The purchase of centrifuges and rotors, power supplies for electrophoresis experiments, fluorometers for DNA quantitation, incubators and baths for enzyme reactions allow this laboratory to be taught to biology majors and medical technology students. The equipment is also used by students enrolled in honors program research or independent study. The university will contribute an amount equal to the award.

由于缺乏分子遗传学的实验室经验， 通过开发分子生物学实验课程来补救 与分子生物学讲座同时进行 成分 实验室经验包括DNA提取和 血浆分离，DNA检测和定量方法， 限制性内切酶，限制性连接 片段，基因标记选择程序，琼脂糖凝胶 电泳和DNA转移和杂交方法。 的 采购离心机和转子， 电泳实验，DNA定量的荧光计， 用于酶反应的培养箱和浴槽使得该实验室 生物学专业和医学技术专业的学生。 参加荣誉课程的学生也使用该设备 研究或独立研究。 该大学将提供一个 金额相当于奖金。