Presidential Young Investigator Award

一个主要的尚未回答的问题，关于 蛋白质复合物合成与生物学功能 这些复合物是如何组装成功能性的 活跃的实体。 为了解决这个问题， 光系统II（PS II），方便的转化系统， 利用蓝细菌集胞藻属PCC 6803 创造了许多PS II突变体， 突变。 这样的突变体提供了一个极好的实验系统 以确定辅助因子（特别是醌类和 叶绿素）在PS II组装和稳定性中起作用。 已知 从其他系统的叶绿素的存在是一个 叶绿素结合蛋白稳定性的要求， 而在高等植物中， PS II中的结合似乎取决于 叶绿素 因此，假定的叶绿素突变- 现在引入结合残基来研究对 叶绿素结合蛋白的合成及稳定性 以及整个PS II综合体。 与总统青年研究者奖，研究的 光系统II（PS II）的合成和功能 光合作用机构正在显着增强，因此， 有助于全面了解其参与 光合作用

One of the major as yet unanswered questions with respect to synthesis and function of protein complexes in biological membranes is how these complexes assemble into functionally active entities. To address this question with respect to photosystem II (PS II), the convenient transformation system of the cyanobacterium Synechocystis sp. PCC 6803 is being utilized to create a number of PS II mutants containing site-directed mutations. Such mutants offer an excellent experimental system to determine the role cofactors (particularly quinones and chlorophylls) play in PS II assembly and stability. It is known from other systems that the presence of chlorophyll is a requirement for stability of chlorophyll-binding proteins, whereas in higher plants synthesis of one of the chlorophyll- binding in PS II appears to depend on the availability of chlorophyll. For this reason, mutations in presumed chlorophyll- binding residues are now introduced to investigate the effects on the synthesis and stability of the chlorophyll-binding proteins as well as of the entire PS II complex. With the Presidential Young Investigator Award, the study of the synthesis and function of photosystem II (PS II) of the photosynthetic apparatus is being significantly enhanced, thus contributing to a comprehensive insight into its involvement in photosynthesis.