A DNA Sequencing Facility for Interdisciplinary Biological Research

用于跨学科生物学研究的 DNA 测序设备

• 批准号: 9419487
• 负责人: Willem Vermaas
• 金额: $ 9.37万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-15 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9419487&HistoricalAwards=false
• 关键词: DNA; Sequencing; Facility; Interdisciplinary; Biological

DNA sequence determinations are of fundamental importance for studies in many areas of the biosciences. including functional biochemistry, cell and molecular biology, population genetics, and molecular systematics. The purpose of this proposal is to streamline DNA sequencing at Arizona State University by seeking funds to establish a DNA sequencing facility. The major piece of equipment for this facility will be an automated DNA sequencer, which currently is not available at our University and which is requested in this proposal. About 20 groups at Arizona State University routinely use DNA sequencing in their research, and a large number of graduate students, undergraduates, postdoctoral fellows, staff, and faculty will benefit from the establishment of a DNA sequencing facility with an automated sequencer. The sequencer requested utilizes an infrared laser diode to excite fluorophor-labeled DNA that is size-separated by gel electrophoresis. Fluorescence from the fluorophor is detected, and simultaneous scanning of four sequencing lanes over time can provide a DNA sequence of 800 nucleotides or more with over 99% accuracy in a highly cost-effective manner. With the equipment requested, 22 samples can be sequenced simultaneously. Apart from providing a 30% cost-share in purchasing this equipment, the University also will create a staff-level position for an individual to run the DNA sequencing facility and will bear most of the personnel cost involved. A Ph.D.-level research scientist (already on staff) will supervise the facility. Nominal user fees (about $5 per sequence) will be charged to cover the cost of chemicals, the DNA sequencer service contract, and a small part (about 20%) of the technician's salary. This facility will provide a highly effective and economic mechanism to obtain and analyze DNA sequence information. An additional benefit is that DNA sequencing becomes accessible to a large number of graduate students who work in research areas (for examp le, systematics and population biology) that would benefit from DNA analysis, but for whom DNA sequencing facilities currently are not readily available. Also, many undergraduate projects, which usually preclude use of radioactivity, could include DNA sequencing. Another significant advantage of acquisition of an automated DNA sequencer is that when DNA sequencing becomes more efficient, students will be able to gather and analyze sequence information more expediently, leaving more room for emphasis on training in other important areas.

DNA序列测定对于生物科学的许多领域的研究具有根本的重要性。包括功能生物化学、细胞和分子生物学、群体遗传学和分子系统学。这项提案的目的是通过寻求资金建立一个DNA测序设施来简化亚利桑那州立大学的DNA测序。该设施的主要设备将是一台自动DNA测序仪，目前我们大学还没有，本提案中要求使用。亚利桑那州立大学的大约20个小组在他们的研究中经常使用DNA测序，大量的研究生、本科生、博士后研究员、工作人员和教师将从建立一个带有自动测序仪的DNA测序设施中受益。 所要求的测序仪利用红外激光二极管激发荧光团标记的DNA，该DNA通过凝胶电泳进行大小分离。 检测来自荧光体的荧光，并且随着时间的推移同时扫描四个测序泳道可以以高度成本效益的方式提供800个核苷酸或更多的DNA序列，具有超过99%的准确度。使用所要求的设备，可以同时对22个样品进行测序。除了提供30%的费用分摊购买这些设备，大学还将创造一个工作人员级别的职位，为个人运行DNA测序设施，并将承担大部分所涉及的人员费用。博士学位-一级研究科学家（已在工作人员）将监督该设施。将收取象征性的用户费用（每个序列约5美元），以支付化学品、DNA测序仪服务合同和技术人员工资的一小部分（约20%）。该装置将为DNA序列信息的获取和分析提供一种高效、经济的机制。另一个好处是，DNA测序变得可用于大量研究生，他们在研究领域（例如，系统学和种群生物学）工作，这些研究领域将受益于DNA分析，但对于他们来说，DNA测序设施目前还不容易获得。此外，许多通常排除使用放射性的本科项目可能包括DNA测序。购买自动DNA测序仪的另一个显著优势是，当DNA测序变得更有效时，学生将能够更方便地收集和分析序列信息，从而为强调其他重要领域的培训留下更多空间。