X-ray Determination of Protein and Viruses

用X射线衍射法研究了各种RNA和DNA动植物病毒的结构、功能和进化关系。最近，罗斯曼博士解决了单链DNA病毒(犬细小病毒)的第一个结构。他现在希望利用这种结构来研究这样的功能，例如，它表面的峡谷状凹陷(这是受体附着的位置吗？)，三重轴上的尖峰(这是中和抗体附着的位置吗？)，五重圆柱形结构(这是将病毒运送到细胞核所必需的)还是部分与衣壳蛋白结合的单链DNA的结构(病毒外壳蛋白能识别特定的DNA序列吗？)罗斯曼博士还在研究单链DNA细菌病毒x174和其他结构，如花椰菜花叶病毒、两种dsRNA病毒(呼肠孤病毒和轮状病毒)以及辛德比斯病毒的核心。主要技术将依赖于在康奈尔高能同步加速器源上快速收集X射线衍射数据，在普渡公司的Cyber 205超级计算机上进行数据处理，主要利用非晶体对称性进行相移，并在交互式计算机图形系统上进行建模和分析。

The structure, function and evolutionary relationship of various RNA and DNA plant and animal viruses are to be studied by use of X-ray diffraction methods. Recently, Dr. Rossmann solved the first structure of a single-stranded DNA virus (canine parvovirus). He now wishes to exploit this structure to investigate such functions as, for instance, the canyon-like depression on its surface (is it the site of receptor attachment?), the spikes on the threefold axes (is this the site where neutralizing antibodies attach?), the fivefold cylindrical structure (is this required for transport of the virus to the cell nucleus) or the structure of part of the ssDNA bound to the capsid proteins (does the viral coat protein recognize a specific DNA sequence?) Dr. Rossmann is also studying the ssDNA bacterial virus X174 and other structure such as those of the cauliflower mosaic virus two dsRNA viruses (reo- and rotaviruses) and the core of Sindbis virus. The primary techniques will depend on fast X-ray diffraction data collection at the Cornell High Energy Synchrotron Source, data processing on Purdue's Cyber 205 supercomputer, phasing primarily by use of the noncrystallographic symmetry and model building and analysis on interactive compute graphics systems.