Biosynthesis of Plant and Algal Tetrapyrrole Pigment Precursors

该项目的重点是了解的重要方面， 作为基本组分的四吡咯颜料的合成 在光合作用和呼吸过程中， 其他重要细胞反应中的辅因子。 的一个重点 生物合成途径涉及中间体5-氨基 乙酰丙酸，它是参与合成乙酰丙酸的酶。 将在物理和动力学方面表征的化合物 性质，辅因子要求，反应机制，可能的 相互作用，以及影响其合成和活性的因素。 分子探针将被生成并用于研究 生物体适应过程中相关基因的表达 光和发展的变化。

This project is focused on understanding important aspects of synthesis of tetrapyrrole pigments that are essential components in the processes of photosynthesis and respiration and are cofactors in other important cellular reactions. A key point in the biosynthetic pathway involves the intermediate, 5-amino levulinic acid, and it is the enzymes involved in synthesis of this compound that will be chacterized in terms of physical and kinetic properties, cofactor requirements, reaction mechanisms, possible interactions, and factors affecting their syntheses and actvities. Molecular probes will be generated and used to study regulation of expression of the relevant genes during adaptation of the organisms to light and developmental changes.