Generation of Mosaicism in Mice by a Site-Specific Recombinase (FLP)

转基因小鼠将在胰岛素或hox启动子的引导下，使用位点特异性酵母重组酶（FLP）在特定细胞中永久激活β -半乳糖苷酶的表达。那些在神经系统发育过程中短暂表达胰岛素或hox的细胞及其后代将被标记。该方法还具有将FLP位点引入细胞的潜力，以便将DNA整合到特定细胞类型的预定染色体位置。通过使用各种启动子，该方法在绘制发育过程中的基因表达模式以及研究基因缺失和基因功能的影响方面具有巨大的潜力。用这种技术培育的转基因小鼠对于研究神经系统特异性和功能的发展将特别有价值。

Transgenic mice will be made using a site-specific yeast recombinase, FLP, under direction of either the insulin or the hox promoter to permanently activate expression of beta-galactosidase in specific cells. Those cells, as well as their progeny, that transiently express insulin or hox during development of the nervous system will be marked. This method also has the potential for introducing an FLP site into cells so that DNA an be integrated into predetermined chromosomal locations in specific cell types. Through the use of various promoters, this method has enormous potential for mapping gene expression patterns during development and for studying effects of gene deletion and gene function. Transgenic mice developed with this technology will be particularly valuable to studies of development of specificity and function in the nervous system.