Periplasmic Cyclic Adenosine Monophosphate Phosphodiesterasefrom Vibrio fischeri

费氏弧菌周质环单磷酸腺苷磷酸二酯酶

• 批准号: 9104653
• 负责人: Paul Dunlap
• 金额: $ 20万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-15 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9104653&HistoricalAwards=false
• 关键词: Periplasmic; Cyclic; Adenosine; Monophosphate; Phosphodiesterasefrom

Vibrio fischeri is a marine bacterium that forms a symbiotic relationship in the light organs of monocentrid fish. A recent finding of the PI reveals that the bacterium produces a phosphodiesterase (CDPase) for cyclic AMP that is of unusual activity and specificity, and is periplasmic in location. The biochemical characteristics, cellular regulation, and functional role of this activity will be examined by a wide spectrum of analytical approaches, including gene cloning and replacement methods, in order to assess the possibility of nucleotide-based biochemical interactions between these symbiotic bacteria and their hosts.//

费氏弧菌是一种海洋细菌，与单中心鱼的发光器官形成共生关系。 PI 最近的一项发现表明，该细菌产生一种针对环 AMP 的磷酸二酯酶 (CDPase)，该酶具有不寻常的活性和特异性，并且位于周质中。 该活动的生化特征、细胞调节和功能作用将通过广泛的分析方法进行检查，包括基因克隆和替换方法，以评估这些共生细菌与其宿主之间基于核苷酸的生化相互作用的可能性。//