Mechanism of Genomic Reprogramming at the Onset of Embryogeny

胚胎发生时基因组重编程的机制

• 批准号: 9105603
• 负责人: Z. Renee Sung
• 金额: $ 18.5万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105603&HistoricalAwards=false
• 关键词: Mechanism; Genomic; Reprogramming; Onset; Embryogeny

The plasticity of the plant genome permits the tissue to change from one form of development to another. Once the developmental fate is determined, a series of morphological changes result that are accompanied by sequential changes in gene expression. The mechanism of the developmental switch is poorly understood, except that major genomic reprogramming might occur. Recently, the PI found that late embryogenesis abundant (LEA) genes are activated at the onset of embryogeny from the zygote, from cultured microspores and from cultured leaf tissue. This result suggests that the embryonic switch involves a global derepression or activation of a battery of genes including both early and late embryonic genes. To substantiate this hypothesis, the PI proposes to study the mechanisms of gene expression at the onset of embryogenesis. Experiments are designed to compare the mechanisms by which a LEA gene, DC8, is expressed at early and late embryogeny, and to identify the genes involved in the activation of the DC8 promotor.

植物基因组的可塑性允许组织从一种发育形式转变为另一种形式。一旦决定了发育的命运，就会产生一系列的形态变化，并伴随着基因表达的顺序变化。除了可能发生重大的基因组重编程外，发育转换的机制还知之甚少。最近，PI发现晚期胚胎发生丰富(LEA)基因在受精卵、培养的小孢子和培养的叶片组织中在胚胎发生开始时被激活。这一结果表明，胚胎转换涉及包括早期和晚期胚胎基因在内的一系列基因的全局去抑制或激活。为了证实这一假设，PI建议研究胚胎发育初期基因表达的机制。实验旨在比较LEA基因DC8在胚胎发育早期和晚期的表达机制，并确定参与DC8启动子激活的基因。