EMF-mediated epigenetic mechanisms in Arabidopsis

拟南芥中电磁场介导的表观遗传机制

• 批准号: 0956409
• 负责人: Z. Renee Sung
• 金额: $ 42万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0956409&HistoricalAwards=false
• 关键词: EMF; mediated; epigenetic; mechanisms; Arabidopsis

PI: Z. Renee Sung, co-PI Daniel ZilbermanIOS-0956409EMF-mediated epigenetic mechanisms in ArabidopsisEMF genes encode chromatin proteins that are master regulators of Arabidopsis development, including the regulation of flowering time. This project studies how the two EMF genes keep plants from flowering. Maintaining floral repression is critical for vegetable crops such as cabbage. A genome-wide approach employing ChIP-chip technology will enable comprehensive identification of EMF/PcG target genes and characterization of the roles of EMF1 and EMF2 in establishing histone methylation (H3K27me3) and transcriptional regulation. A molecular genetic approach will be employed to investigate the function of EMF1-target gene interaction. This project will uncover the EMF-regulated genetic network that controls vegetative development, and the role EMF1 plays in epigenetic regulation of gene expression. Data will be made available to the public via Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/), a curated online resource maintained by NCBI. The proposed research will provide training opportunities in developmental genetics, chromatin biology and genomics for a postdoctoral fellow and student researchers, including students from underrepresented and disadvantaged backgrounds.

emf基因编码的染色质蛋白是拟南芥发育的主要调控因子，包括开花时间的调控。本项目研究两种EMF基因如何阻止植物开花。维持花的抑制对卷心菜等蔬菜作物至关重要。采用ChIP-chip技术的全基因组方法将能够全面鉴定EMF/PcG靶基因，并表征EMF1和EMF2在建立组蛋白甲基化（H3K27me3）和转录调控中的作用。将采用分子遗传学的方法来研究emf1 -靶基因相互作用的功能。本项目将揭示emf调控的控制营养发育的遗传网络，以及EMF1在基因表达的表观遗传调控中的作用。数据将通过Gene Expression Omnibus （http://www.ncbi.nlm.nih.gov/geo/）向公众提供，这是一个由NCBI维护的策划在线资源。拟议的研究将为博士后和学生研究人员提供发育遗传学、染色质生物学和基因组学方面的培训机会，包括来自代表性不足和弱势背景的学生。