Understand and development of electroporation for biologicalresearch

生物研究中电穿孔的理解和发展

• 批准号: 9106103
• 负责人: Donald Chang
• 金额: $ 10万
• 依托单位: Marine Biological Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-11-15 至 1995-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9106103&HistoricalAwards=false
• 关键词: Understand; development; electroporation; biologicalresearch

Recently, a new technique was developed which utilizes high intensity electrical pulses to temporarily permeabilize the cell membrane, a process called "electroporation". With this method, exogenous molecules (including proteins, antibodies and genes) can be introduced into living cells. The major objectives of this research are: (1) To obtain a better understanding of the basic processes and mechanisms of electroporation. (2) To improve the technology electroporation and to develop more widely useful instrumentation. (3) To develop new applications of electroporation for research in cell biology, molecular biology, developmental biology and neurobiology. The technology developed in this laboratory will be made available to other scientists through the establishment of a collaborative facility located at the Marine Biological Laboratory, Wood Hole, MA. A number of collaborative studies using an improved method of electroporation have been planned.

最近，一种利用高强度电脉冲暂时渗透细胞膜的新技术被开发出来，这一过程被称为电穿孔。通过这种方法，可以将外源分子(包括蛋白质、抗体和基因)引入活细胞。本研究的主要目的是：(1)更好地了解电穿孔的基本过程和机理。(2)改进电穿孔技术，开发更广泛的实用仪器。(3)开发电穿孔技术在细胞生物学、分子生物学、发育生物学和神经生物学研究中的新应用。该实验室开发的技术将通过在马萨诸塞州伍德霍尔海洋生物实验室建立合作设施，供其他科学家使用。已经计划了一些使用改进的电穿孔方法的合作研究。