Regulatory Mechanisms of Cucumber Mosaic Virus Replication

黄瓜花叶病毒复制的调控机制

• 批准号: 9106293
• 负责人: Peter Palukaitis
• 金额: --
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-15 至 1994-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9106293&HistoricalAwards=false
• 关键词: Regulatory; Mechanisms; Cucumber; Mosaic; Virus

The subject of this proposal is cucumber mosaic virus (CMV), a wide host-range plant virus for which there is very little natural resistance available. The goals of this project are: (1) to localize sequences in the viral genome involved in the regulation of the rate of replication, and the inhibition of replication by high temperatures; (2) to determine the underlying mechanisms of differential replication in plant cells (protoplast) affected by various genetic alterations; and (3) to determine the regulatory effects of various genetic alterations on the viral replicase in vitro. These goals will be achieved by using biologically-active DNA clones of strains of CMV, that show differences in the regulation various aspects of CMV (and satellite) RNA replication in several cucurbit hosts. The sequences controlling the various replicate-related phenotypes will be mapped by recombination and site-directed mutagenesis of the DNA clones. The specific functions altered by these sequence substitutions will be determined by analyses of the replication products both in in vitro infected protoplasts, as well as of the purified viral replicase provided with the appropriate templates and incubated under the experimental conditions. These analyses will relate differences in viral protein structure to specific functions.***//

该提案的主题是黄瓜花叶病毒（CMV），一种广泛的 宿主范围植物病毒，其在自然界中很少 阻力可用。 本项目的目标是：（1） 定位病毒基因组中参与调控的序列 复制的速度，以及复制的抑制， 高温;（2）确定潜在的机制， 影响植物细胞（原生质体）的差异复制 各种遗传改变;（3）确定调节 各种遗传改变对病毒复制酶的影响， 体外 这些目标将通过使用生物活性 CMV株系的DNA克隆，其显示了在 调控CMV（和卫星）RNA复制的各个方面 在几个葫芦科寄主中 控制各种 将通过重组绘制复制相关表型， DNA克隆的定点诱变。 具体 由这些序列取代改变的功能将被 通过分析体外和体外的复制产物确定 感染的原生质体，以及纯化的病毒复制酶 提供适当的模板，并在 实验条件 这些分析将把差异 在病毒蛋白质结构中的特定功能。*//