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Analysis of virus resistance mechanisms in transgenic tobacco plants expressing the coat protein gene of cucumber mosaic virus

表达黄瓜花叶病毒外壳蛋白基因的转基因烟草植物抗病毒机制分析

基本信息

批准号：
04660050
负责人：
OKONO Teturou
金额：
$ 1.34万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Resistance levels against infection with cucumber mosaic virus (CMV) of transgenic tobacco plants expressing the coat protein gene of CMV (CP+) were influenced by several factors as followings.1) At elevated temperature (30-32 C), CP+ became susceptible to infection with CMV.Accumulation levels of endogenous coat protein in CP+ decreased at 30-32 C compared at normal temperature (23-27 C) although accumulation of coat protein mRNA was not so much influenced by temperature. This suggested that coat protein was more directly involved in virus resistance of CP+ plants than mRNA of the gene.2) CP+ showed differential susceptibility to infection with different strains of CMV containing no satellite RNA (sat-RNA). CP+ was more susceptible to the F and the P strains of CMV than to the Y strain of CMV whose coat protein gene was used to obtain transgenic tobacco plants.3) CP+ was more susceptible to infection with sat-RNA-free CMV than CMV containing Sat-RNA in any strain of CMV tested here.4) CP+ was more susceptible to infection with psedorecombinants containing RNA3 of either the Y strain or the F strain and RNA1 and 2 of the F strain and the Y strain, respectively, than to infection with the Y strain. The results obtained here suggest that coat protein of inoculum virions was not a direct determinant for virus resistance in CP+ and that the CMV resistance in CP+ was influenced by biological properties of CMV including interaction of RNA1, 2, and 3 and their products. Mechanisms of CMV coat protein-mediated protection are more complex.

表达黄瓜花叶病毒（CMV）外壳蛋白基因（CP+）的转基因烟草对CMV感染的抗性水平受以下几个因素的影响：1）在高温（30-32 ℃）下，与常温（23-27 ℃）相比，30-32 ℃时CP+的内源外壳蛋白积累水平降低而温度对外壳蛋白mRNA的积累影响不大。CP+对不含卫星RNA（sat-RNA）的不同CMV株系的侵染表现出不同的敏感性。CP+对CMV的F、P株系的感病性高于利用外壳蛋白基因获得转基因植株的CMV的Y株系; 3）CP+对不含Sat-RNA的CMV的感病性高于含Sat-RNA的CMV对分别含有Y株或F株的RNA 3以及F株和Y株的RNA 1和RNA 2的假重组体的感染更敏感，而不是Y病毒株的感染结果表明，接种病毒的外壳蛋白不是CP+中病毒抗性的直接决定因素，CP+中CMV抗性受CMV生物学特性的影响，包括RNA 1、2和3与其产物的相互作用。CMV外壳蛋白介导的保护机制更为复杂。