Signal Transduction in Plant Guard Cells

植物保卫细胞将用于研究细胞过程 植物细胞膜的信号转导。 保卫细胞 将环境刺激转化为膨压的变化 与质子泵活性有关。 该项目将研究 生电泵介导保卫细胞反应的机制 光和植物激素的刺激。 电生理 使用膜片钳技术的方法将用于测量 原生质体和液泡中的产电泵电流。 荧光探针结合视频成像技术 将用于检查内部pH值的变化， 质子泵 研究的重点是：1） 通过红色和蓝色光感受控制质子泵电流，2） 植物激素如脱落酸和吲哚乙酸的影响 酸对产电泵电流的影响，3）质子泵的调节 电流的细胞质因子，4）内部pH值的变化， 伴随质子泵的刺激，以及5） 液泡膜中的质子泵电流。 这项研究将使用电生理学技术， 荧光探针结合视频成像技术， 光学显微镜提供了关于 植物膜传递环境信号的机制 细胞反应，以及钙和pH值所起的作用 在信号传导过程中的作用

Plant guard cells will be used to study cellular processes underlying signal transduction in plant membranes. Guard cells transduce environmental stimuli into changes in turgor pressure associated with proton pump activity. This project will examine mechanisms by which electrogenic pumps mediate guard cell responses to stimuli such as light and plant hormones. Electrophysiological approaches using the patch clamp technique will be used to measure electrogenic pump currents in protoplasts and vacuoles. Fluorescent probes in conjunction with video imaging techniques will be used to examine changes in internal pH that accompany proton pumping. Research will focus on the study of: 1) the control of proton pump currents by red and blue photoreception, 2) effects of plant hormones such as abscisic acid and indole acetic acid on electrogenic pump currents, 3) regulation of proton pump currents by cytoplasmic factors, 4) changes in internal pH that accompany stimulation of proton-pumping, and 5) the properties of proton pump currents in the vacuolar membrane. This research will use techniques of electrophysiology and fluorescent probes in combination with video imaging techniques for the light microscope to provide fundamental new information on the mechanisms by which plant membranes transduce environmental signals into cellular responses, and on the role played by calcium and pH in the chain of signal transduction events.