Signal Transduction in Plant Guard Cells

植物保卫细胞中的信号转导

基本信息

  • 批准号:
    9106515
  • 负责人:
  • 金额:
    $ 47.83万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    1991
  • 资助国家:
    美国
  • 起止时间:
    1991-08-15 至 1998-07-31
  • 项目状态:
    已结题

项目摘要

Plant guard cells will be used to study cellular processes underlying signal transduction in plant membranes. Guard cells transduce environmental stimuli into changes in turgor pressure associated with proton pump activity. This project will examine mechanisms by which electrogenic pumps mediate guard cell responses to stimuli such as light and plant hormones. Electrophysiological approaches using the patch clamp technique will be used to measure electrogenic pump currents in protoplasts and vacuoles. Fluorescent probes in conjunction with video imaging techniques will be used to examine changes in internal pH that accompany proton pumping. Research will focus on the study of: 1) the control of proton pump currents by red and blue photoreception, 2) effects of plant hormones such as abscisic acid and indole acetic acid on electrogenic pump currents, 3) regulation of proton pump currents by cytoplasmic factors, 4) changes in internal pH that accompany stimulation of proton-pumping, and 5) the properties of proton pump currents in the vacuolar membrane. This research will use techniques of electrophysiology and fluorescent probes in combination with video imaging techniques for the light microscope to provide fundamental new information on the mechanisms by which plant membranes transduce environmental signals into cellular responses, and on the role played by calcium and pH in the chain of signal transduction events.
植物保卫细胞将用于研究细胞过程 植物细胞膜的信号转导。 保卫细胞 将环境刺激转化为膨压的变化 与质子泵活性有关。 该项目将研究 生电泵介导保卫细胞反应的机制 光和植物激素的刺激。 电生理 使用膜片钳技术的方法将用于测量 原生质体和液泡中的产电泵电流。 荧光探针结合视频成像技术 将用于检查内部pH值的变化, 质子泵 研究的重点是:1) 通过红色和蓝色光感受控制质子泵电流,2) 植物激素如脱落酸和吲哚乙酸的影响 酸对产电泵电流的影响,3)质子泵的调节 电流的细胞质因子,4)内部pH值的变化, 伴随质子泵的刺激,以及5) 液泡膜中的质子泵电流。 这项研究将使用电生理学技术, 荧光探针结合视频成像技术, 光学显微镜提供了关于 植物膜传递环境信号的机制 细胞反应,以及钙和pH值所起的作用 在信号传导过程中的作用

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Elba Serrano其他文献

Experimental investigation of effects of indentation depth and probe tip radius on apparent young's modulus of Xenopus laevis oocytes
  • DOI:
    10.1016/j.bpj.2022.11.2827
  • 发表时间:
    2023-02-10
  • 期刊:
  • 影响因子:
  • 作者:
    Tatiana Kardashina;Elba Serrano;Charles B. Shuster;Borys Drach
  • 通讯作者:
    Borys Drach

Elba Serrano的其他文献

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{{ truncateString('Elba Serrano', 18)}}的其他基金

MRI: Acquisition of a Bio-AFM for Life Science and Soft Matter Research and Research Training in New Mexico
MRI:在新墨西哥州购买用于生命科学和软物质研究和研究培训的生物原子力显微镜
  • 批准号:
    2117045
  • 财政年份:
    2021
  • 资助金额:
    $ 47.83万
  • 项目类别:
    Standard Grant
Resource Hub: The NSF National Resource Hub for STEM Education at Hispanic-Serving Institutions
资源中心:NSF 拉美裔服务机构 STEM 教育国家资源中心
  • 批准号:
    1832338
  • 财政年份:
    2018
  • 资助金额:
    $ 47.83万
  • 项目类别:
    Standard Grant
Bridging Biology and Physics Undergraduate Education through Nanoscience: A New Mexico Network Approach
通过纳米科学架起生物学和物理学本科教育的桥梁:新墨西哥州网络方法
  • 批准号:
    0633736
  • 财政年份:
    2007
  • 资助金额:
    $ 47.83万
  • 项目类别:
    Standard Grant
U.S.-Brazil Cooperative Research: Scientific visit to Plan aProject on Signal Transduction in Tropical C3-CAM Plants; Brasilia, Brazil; August 14-28, 1992
美国-巴西合作研究:对热带 C3-CAM 植物信号转导计划项目进行科学访问;
  • 批准号:
    9220300
  • 财政年份:
    1992
  • 资助金额:
    $ 47.83万
  • 项目类别:
    Standard Grant

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