Numerical Instrumentation for Shape and Shape Change Analysis via Three-Dimensional Microscopy

研究人员将把研究重点放在充分开发 显微系统的自动化仪器能力 提供了量化三维形状的可能性， 参数，以及形状参数随时间的变化， 微观生物标本。 他们计划继续 他们研究互补成像多样性的项目 模式，包括共聚焦显微镜，立体显微镜， 常规光学切片显微镜。 学习计划 将包括通过各种方法的微观数据采集。 所获得的图像将经过适当的过滤 这些方案基于成像的物理原理， 锐化并提高单个显微照片的图像清晰度， 为了去除对本地样本的信息的虚假信息， 结构 过滤技术，这将需要 在其发展过程中进行了大量的分析研究， 具体到所使用的每个光学系统。最后，总 本地光学数据将通过 复杂的数字仪器技术来重建 一个给定的成像标本的三维结构，并量化其 随着时间的推移，它的大小和形状特征。 的 开发的方法将在广泛的 一系列标本类型，包括染色和未染色的单个 和多细胞实验对象 几种不同的组织 制备将用于研究样品的时间演化 在各种生物过程中随时间变化的形态。

The investigators will focus their research on fully developing the automated instrumentation capabilities of microscopic systems that offer the potential for quantifying the 3-D shape parameters, and changes in shape parameters over time, of microscopic-scale biological specimens. They plan to continue their program of studying diversity of complementary imaging modalities, including confocal microscopy, stereo microscopy, and conventional optical sectioning microscopy. The program of study will encompass microscopic data acquisition via various methods. The images obtained will be subjected to appropriate filtering schemes base on the physical principles of image formation to sharpen and improve image clarity in individual micrographs and to remove information spurious to that of the local specimen structure. The filtering techniques, which will require considerable analytic study in their development, will be specific to each optical system used. Finally, the aggregate local optical data will be assembled and analyzed via sophisticated numerical instrumentation techniques to reconstruct the 3-D structure of a given imaged specimen and to quantify its size and shape characteristics as it evolves over time. The methods developed will be implemented and evaluated on a broad range of specimen types, including stained and unstained single and multiple celled subjects. Several different tissue preparations will be used to study the time evolution of specimen morphology over time during various biological processes.