MRI: Track 1 Acquisition of Instrumentation for Marine Metal-Organic and Metalloproteomic Analyses

MRI：第 1 轨道采购海洋金属有机和金属蛋白质组分析仪器

• 批准号: 2320496
• 负责人: Mak Saito
• 金额: $ 99.98万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2026-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2320496&HistoricalAwards=false
• 关键词: MRI; Track; Acquisition; Instrumentation; Marine

In environmental sciences, the organic and inorganic components of natural molecules have generally been considered separately. Yet it has become clear in recent decades that the organic-inorganic interface is critically important in biological systems: from the inorganic perspective, there are essential uses of metals in all life forms due to the unique chemical properties metal ions provide. For example, iron and manganese are essential in photosynthesis, copper, iron, and molybdenum are co-factors in multiple key nitrogen cycle reactions, and cobalt, zinc, and even cadmium are critical to carbon uptake and intracellular transformations. And from the organic perspective these metals are typically bound by a wide variety of organic molecules. This project will support the acquisition of a modern mass spectrometer system that contribute to a system capable of interrogating both the organic and inorganic chemical complexities. The instrumentation will contribute to understanding of ocean biogeochemical cycling through the analysis of large, coordinated sampling efforts such as GEOTRACES (including in the upcoming Antarctic GP17 expedition), AtlantEco, and other collaborative projects, measuring metal-ligands and microbial proteins across large regions of the oceans. The proposed instrumentation will also benefit researchers within the ocean science community through a variety of collaborative studies including an ongoing collaboration with a HBCU, as well as through the dissemination of novel organic-inorganic methods. Data produced by this mass spectrometer will be shared on public repositories and incorporated into the Ocean Protein Portal, a web resource that makes ‘omic data accessible to non-expert users used in both research and education activities including class-based research projects. The hybrid instrument developed in this project will enable interdisciplinary inorganic-organic studies. Specifically, the acquired orbitrap mass spectrometry instrumentation will be coupled to an inorganic plasma mass spectrometer via a custom multidimensional chromatographic system allowing simultaneous characterization of complex organic molecules and their metal contents. The resulting instrumentation will enable advances in areas of metal-ligand and dissolved organic matter characterization, metalloproteomics, and metaproteomics and will enhance new collaborative research studies on marine bioinorganic chemistry in the ocean sciences. Knowledge gained in these areas will improve the understanding of controls on Earth’s biogeochemical cycles through the study of nutrient stress biomarkers, biogeochemically relevant (metallo)enzymes, and metal cycling and bioavailability.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在环境科学中，通常已经分别考虑了天然分子的有机和无机成分。然而，最近几十年来，有机无机界面在生物系统中至关重要：从无机的角度来看，由于金属离子提供的独特化学性质，所有生命形式都有金属的必要用途。例如，铁和锰在光合作用，铜，铁和钼中至关重要，是多个关键的氮循环反应中的副因素，钴，锌甚至镉对碳吸收和细胞内转化至关重要。从有机的角度来看，这些金属通常由多种有机分子结合。该项目将支持对现代质谱仪系统的获取，该系统有助于能够询问有机和无机化学复杂性的系统。该仪器将通过分析大型，协调的抽样工作（包括在即将到来的南极GP17探险中），Atlanteco和其他协作项目，有助于了解海洋生物地球化学循环。拟议的仪器还将通过各种协作研究，包括与HBCU进行的合作以及通过传播新颖的有机无机方法的持续合作，使海洋科学界的研究人员受益。该质谱仪生产的数据将在公共存储库中共享，并将其纳入Ocean Protein Portal，这是一种Web资源，它使得在研究和教育活动中使用的非专家用户可以访问包括基于课堂的研究项目的“ OMIC数据”。该项目中开发的混合仪器将实现跨学科无机有机研究。具体而言，获得的Orbitrap质谱仪器将通过自定义的多维色谱系统耦合到无机等离子体质谱仪，从而可以简单地表征复杂的有机分子及其金属含量。最终的仪器将能够在金属配体和溶解有机物表征，金属蛋白质组学和元蛋白质组学领域的进步，并将增强有关海洋科学海洋生物促进化学的新合作研究。通过研究养分应力生物标志物，生物地球化学相关（金属）酶以及金属循环和生物利用度，这将在这些领域获得的知识将改善对地球生物地球化学循环的控制的理解。该奖项反映了NSF的法定任务，并通过评估了基金会的范围来反映出支持的人，并在宽广的知识上进行了支持。