The Enzymology and Regulation of Nitrate Assimilation

硝酸盐同化的酶学和调节

• 批准号: 9107561
• 负责人: Reginald Garrett
• 金额: $ 11万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-15 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9107561&HistoricalAwards=false
• 关键词: Enzymology; Regulation; Nitrate; Assimilation

Nitrate assimilation in the fungus Neurospora, particularly the steps connected with nitrite assimilation and reduction to ammonium ion, is the object(s) of the proposed research. An analysis of the sequence organization and regulated expression of the nitrite reductase structural gene, "nit-6", and a comparison of its properties with those of "nit-3", its coordinately regulated partner, is an immediate goal of the research. The information obtained will be exploited in experiments designed to probe structure:activity relationships in nitrite reductase.

本文拟研究神经孢子菌的硝酸盐同化，特别是与亚硝酸盐同化和还原为铵离子有关的步骤。分析亚硝酸盐还原酶结构基因“niti -6”的序列组织和调控表达，并将其与协同调控的伙伴“niti -3”的性质进行比较，是本研究的直接目标。获得的信息将在实验中被利用，旨在探索亚硝酸盐还原酶的结构：活性关系。