U.S.-Hungary Joint Fund Research on Microspores for Maize Transformation

美国-匈牙利小孢子玉米转化联合基金研究

• 批准号: 9107645
• 负责人: Beata Barnabas
• 金额: --
• 依托单位: Agricultural Research Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-15 至 1995-06-15
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9107645&HistoricalAwards=false
• 关键词: U.S.; Hungary; Joint; Fund; Research

The primary objective of this U.S.-Hungary research project under the Hungary Joint Fund between Dr. Joseph Mascarenhas, State University of New York at Albany and Dr. Beata Barnabas, Agricultural Research Institute of the Hungarian Academy of Sciences, is to develop methods for the production of mature, viable pollen from microspores cultured in anthero. The work will involve exposing microspores by making a cut in the anther wall prior to micro- projectile bombardment and the pollen will then be matured in anthero. If and when this preliminary work indicated that the system seems to be working well, the pollen matured in anthero will be used for pollination to study stable transformation of maize plants. Maize is a very important agricultural crop both in Eastern Europe and the US and a simple transformation procedure using pollen would be of significance for basic studies in genetics and developmental biology of the plant and in the engineering of new traits of agronomic value. This project in plant physiology fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents and pool research resources in areas of strong mutual interest and competence.

这个美国-匈牙利研究项目由纽约州立大学奥尔巴尼分校的 Joseph Mascarenhas 博士和匈牙利科学院农业研究所的 Beata Barnabas 博士之间的匈牙利联合基金资助，其主要目标是开发从花药中培养的小孢子生产成熟、有活力的花粉的方法。 这项工作将包括在微射弹轰击之前在花药壁上切开一个切口来暴露小孢子，然后花粉将在花药中成熟。 如果这项初步工作表明该系统似乎运行良好，花药中成熟的花粉将用于授粉，以研究玉米植株的稳定转化。 玉米在东欧和美国都是一种非常重要的农作物，使用花粉的简单转化程序对于植物遗传学和发育生物学的基础研究以及农艺价值新性状的工程具有重要意义。 该植物生理学项目通过使美国和东欧的领先专家能够在共同感兴趣和能力强的领域结合互补人才并汇集研究资源，实现了推进科学知识的计划目标。