U.S.-Hungary Joint Fund Project Development for Research on Receptor Mediated Ion Translocation

美国-匈牙利受体介导离子易位研究联合基金项目开发

• 批准号: 9207396
• 负责人: Julianna Kardos
• 金额: --
• 依托单位: Central Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1993-06-01
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9207396&HistoricalAwards=false
• 关键词: U.S.; Hungary; Joint; Fund; Project

The primary objective of this U.S.-Hungary cooperative research project between Dr. Julianna Kardos of the Central Research Institute for Chemistry, Budapest and Dr. Derek Cash, University of Missouri, Columia is to use fast kinetic technique for the analysis of transmembrane ion fluxes mediated by the ion-channel forming neurotransmitter receptor such as gamma-aminobutyric acid (GABA). Determining the relationship between the binding reaction and the GABA receptor-controlled 36CL -ion fluxes requires the techniqus of rapid mixing because - due to rapid desensitization of receptor on exposure to GABA - the translocation process is complete within a few hundred milliseconds. For the existing fast reacting techniques, Dr. Kardos plans to explore the versatile quenched flow machine developed by Dr. Cash and is now constructing the machine in his laboratory. The project development visit by Prof. Cash would focus on extending their previous work on the GABA receptor to drugs which effect its function using a preparation enriched in GABA receptors. Funds for this project are awarded through the US-Hungary Joint Fund, Budapest in accordance with established guidelines.

布达佩斯中央化学研究所的 Julianna Kardos 博士和密苏里大学哥伦比亚分校的 Derek Cash 博士之间的这个美国-匈牙利合作研究项目的主要目标是使用快速动力学技术来分析由离子通道形成神经递质受体（例如 γ-氨基丁酸 (GABA)）介导的跨膜离子通量。 确定结合反应与 GABA 受体控制的 36CL 离子通量之间的关系需要快速混合技术，因为由于受体暴露于 GABA 后快速脱敏，易位过程在几百毫秒内完成。 对于现有的快速反应技术，卡多斯博士计划探索由卡什博士开发的多功能骤冷流机器，目前正在他的实验室中建造该机器。 Cash 教授的项目开发访问将重点是将他们之前在 GABA 受体方面的工作扩展到使用富含 GABA 受体的制剂来影响其功能的药物。 该项目的资金由布达佩斯的美国-匈牙利联合基金根据既定准则提供。