Insertion Mutagenesis in Arabidopsis: Toward a Standard Population

拟南芥插入诱变：走向标准种群

• 批准号: 9108442
• 负责人: Kenneth Feldmann
• 金额: $ 36.6万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9108442&HistoricalAwards=false
• 关键词: Insertion; Mutagenesis; Arabidopsis; Toward; Standard

Dr. Feldmann will produce unique transformants of Arabidopsis with the seed infection procedure using the T-DNA tagging method. The target is to produce 5,000 lines a year. The transformants will be screened initially on agar plates, and then in soil grown plants. All lines that have a visible alteration in phenotype will be recorded and made available to the general scientific community. The availability of the seeds will be announced through the electronic Arabidopsis bulletin board. Scientists who wish to screen for specific phenotypes will be permitted to come to University of Arizona to do so on a case by case basis. Dr. Feldmann plans to deposit the mutant lines to a national Arabidopsis resource center at the end of this grant period. The importance of seed transformation in the tagging and cloning of Arabidopsis genes have been established. It is extremely time- consuming to produce the tagged lines and this project will provide invaluable resources to the plant molecular biology community.

费尔德曼博士将培育出独特的拟南芥转化体 用T-DNA标记法进行种子感染。 目标是每年生产5,000条线。 转化体 将首先在琼脂平板上筛选，然后在土壤中生长 植物 表型有明显变化的所有品系 将被记录下来并提供给普通科学家， 社区 种子的供应情况将于 通过电子拟南芥公告板。 科学家 希望筛选特定表型的人将被允许 来亚利桑那大学这样做的个案的基础上。 费尔德曼博士计划将变异株存款 拟南芥资源中心在此资助期结束。 的 种子转化在植物标记和克隆中的重要性 拟南芥基因已经建立。 是时候了- 消耗生产标记线，该项目将 为植物分子生物学提供了宝贵的资源 社区