An Ac-based tagging vector for insertional mutagenesis in _ Arabidopsis thaliana

用于拟南芥插入诱变的基于 Ac 的标记载体

• 批准号: 9119526
• 负责人: Barbara Baker
• 金额: $ 27.27万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-15 至 1996-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9119526&HistoricalAwards=false
• 关键词: Ac; based; tagging; vector; insertional

This is a proposal to develop a transposon tagging system for Arabidopsis, based on the maize transposon Activator. It is proposed that an Ac-derived tagging vector will be developed based on the novel concept of a suicide Ac element. Transposition of suicide Ac will be limited to a single event in post-meiotic pollen of a primary transformant, thus individual progeny of the transformant will contain a single element at a unique genomic location. This will allow easy assessment of distribution of element target sites. If successful, the result would be a new and versatile tool with which insertional mutations could readily be produced, analyzed and cloned in Arabidopsis.

这是一个建议，开发一个转座子标记系统， 拟南芥，基于玉米转座子激活子。 建议开发一种Ac衍生的标签载体 基于自杀AC元件的新概念。 换位 自杀Ac将仅限于减数分裂后的单一事件 花粉的初级，因此个别后代的 DNA将在一个独特的基因组中包含一个单一的元素， 位置. 这将使人们能够容易地评估 元素靶位点。 如果成功，结果将是一个新的多功能工具， 哪些插入突变可以容易地被产生、分析和 在拟南芥中克隆。