Effect of Immobilization on Metabolism of Escherichia Coli: Research Planning Grant

固定化对大肠杆菌代谢的影响：研究计划资助

• 批准号: 9111796
• 负责人: Judith Miller
• 金额: $ 1.8万
• 依托单位: Worcester Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9111796&HistoricalAwards=false
• 关键词: Effect; Immobilization; Metabolism; Escherichia; Coli

The use of immobilized whole cells to conduct biochemical conversions has increased significantly in the last several years. One of the most important areas is the effects of immobilization on the intrinsic metabolism of the cells employed. Little has been reported, particularly with respect to E. coli, on how immobilization affects carbon flow, energy production, and product formation. Preliminary results have shown that significant changes in metabolism occur upon immobilization. E. coli cells grown in alginate synthesize significantly more beta-galactosidase per cell, and elevated quantities of the normal metabolic byproducts acetate, pyruvate, and lactate, compared to free cells. These results support the hypothesis that immobilization changes the relative rates of carbon consumption and growth. The specific objectives of this work are to test the following two hypotheses: i) that the excess carbon consumed by immobilized cells is diverted to the synthesis of acid byproducts or of inducible protein; and ii) that mass transfer limitations on the removal of acid byproducts cause the observed changes in metabolism. The ultimate goal is to exploit immobilization-induced metabolic changes for the overproduction of recombinant proteins.

固定化全细胞的应用 进行生化转化 大幅增加 过去几年。一个最 重要领域是影响 内在固定化 所用细胞的代谢。 很少有报道， 特别是对于E. 大肠杆菌，关于固定如何影响 碳流、能源生产， 产物形成 初步 结果显示， 新陈代谢的变化发生在 固定化。 e.杆菌细胞 在海藻酸合成中生长 显著更多 β-半乳糖苷酶/细胞，和 正常水平升高 代谢副产物乙酸盐， 丙酮酸和乳酸， 自由细胞这些结果支持 固定化假设 改变了 碳消费和增长。 这一具体目标 工作是测试以下两个 假设：（一）超额 固定化碳耗 细胞被转移到合成 酸副产物或可诱导的 蛋白质;和ii）质量传递 酸去除的限制 副产品导致观察到的 新陈代谢的变化。 的 最终目的是利用 制动诱导代谢 生产过剩的变化 重组蛋白