Electron Transfer Reactions Between Cytochrome c, Cytochrome c Oxidase and Dioxygen

细胞色素 c、细胞色素 c 氧化酶和双氧之间的电子转移反应

• 批准号: 9111786
• 负责人: Robert Bass
• 金额: $ 39.9万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-15 至 1994-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9111786&HistoricalAwards=false
• 关键词: Electron; Transfer; Reactions; Between; Cytochrome

This research, supported by the Analytical and Surface Chemistry Program in the Chemistry Division, will study electron transfer thermodynamics, kinetics, and mechanisms of enzyme action for cytochrome c oxidase. The enzyme will be adsorbed to a gold electrode and embedded in a lipid bilayer. Through both electrochemical and spectroscopic measurements (including magnetic circular dichroism) the electrical and structural dynamics of oxidation/reduction processes will be studied. The use of lipid bilayers will keep enzyme subunits from clustering at the electrode surface, improving the likelihood that the dynamics observed correspond to the specific units desired rather than to agglomorates. %%% Cytochrome c is a ubiquitous biological transfer agent. The efficiency of respiration and photosynthesis are largely dependent on the dynamics of electron transfer between cytochrome c and two enzymes: cytochrome c oxidase and cytochrome c reductase. This research will study the electron transfer dynamics by adsorbing cytochrome c reductase on a gold electrode, otherwise covered by a lipid bilayer to emulate a cell membrane. The goal of the work is to observe conformation changes in the enzyme as a result of electron transfer; such changes are hypothesized to be an essential feature of efficient biological electron transfer processes.

这项研究得到了分析和表面的支持， 化学系的化学课程，将学习 电子转移的热力学、动力学和机理 细胞色素c氧化酶的酶作用。 这种酶将 吸附到金电极上并嵌入脂质双层中。 通过电化学和光谱测量 （包括磁性圆二色性）的电和 氧化/还原过程的结构动力学将是 研究了 脂质双层的使用将保持酶亚基 在电极表面聚集，提高了 观察到的动态对应于 而不是聚集在一起。 %%% 细胞色素c是一种普遍存在的生物转移剂。 的 呼吸和光合作用的效率在很大程度上 取决于电子在 细胞色素c和两种酶：细胞色素c氧化酶， 细胞色素c还原酶 这项研究将研究电子 金吸附细胞色素c还原酶的传递动力学 电极，否则由脂质双层覆盖以模拟 细胞膜 这项工作的目的是观察构象 由于电子转移而引起的酶的变化;例如 变化被假设为是一个基本特征， 有效的生物电子转移过程。