Electron Transfer Reactions Between Cytochrome c, Cytochrome c Oxidase and Dioxygen

通过含有细胞色素c氧化酶的双层修饰金电极的可重复性制备是本研究项目的目标，由分析和表面化学计划支持。这些电极表面将被设计成在酶和金电极表面之间表现出直接的电子转移活性，并且使用这些修饰的电极表面将探索氧化磷酸化的功能电子转移机制。电子在膜系统中传递的方式对于理解许多生化问题具有根本的重要性。在这个项目模型中，膜结合蛋白系统将附着在金表面，电子转移过程发生在其中，将被广泛表征。这将增加对生物过程的理解，并为电化学生物传感器的合理设计奠定基础。

The reproducible preparation of gold electrodes modified by bilayers containing cytochrome c oxidase is the goal of this research project supported by the Analytical and Surface Chemistry Program. These electrode surfaces will be designed to exhibit direct electron transfer activity between the enzyme and the gold electrode surface, and functional electron transfer mechanisms of oxidative phosphorylation will be probed using these modified electrode surfaces. The way in which electrons are transferred in membrane systems is of fundamental importance to the understanding of many biochemical problems. In this project model membrane bound protein systems will be attached to gold surfaces and the electron transfer processes that occur in them will be extensively characterized. This will both provide an increased understanding of biological processes and will lay the groundwork for the rational design of electrochemical biosensors.