Molecular Cytology Core Facility

Thomas Phillips博士与十个主要用户一起，提议在密苏里大学建立一个免疫细胞化学和原位杂交技术资源中心。该中心将包括一个共聚焦荧光显微镜，一个用于共聚焦显微镜图像三维重建的计算机图形系统，一个用于荧光研究的低照度显微镜和相机，以及一个用于指示染料定量成像以确定细胞pH值或离子浓度的相关图像处理系统。该中心将整合一些现有的设备：透射电子显微镜、显微切片机和低温恒温器。该设施将补充现有的校园范围内的单克隆抗体生产和寡核苷酸合成核心。大学内的各个单位将提供50%的资本成本、员工的长期工资支持、大量现有设备、实验室装修和持续服务合同的资金。

Dr. Thomas Phillips, in conjunction with ten major users, proposes to set up a resource center for immunocytochemistry and in situ hybridization technologies at the University of Missouri. The center would include, and funds are requested for, a confocal fluorescence microscope, a computer graphics system for three-dimensional reconstruction of confocal microscope images, a low-light microscope and camera for fluorescence studies, and an associated image processing system for ration imaging of indicator dyes to determine cellular pH or ion concentrations. The center will incorporate some already existing equipment: a transmission electron microscope, microtomes and a cryostat. This facility would complement existing campus-wide cores for monoclonal antibody production and oligonucleotide synthesis. Various units within the University will provide 50% of the capital costs, long-term salary support for staff, substantial existing equipment, and funding for laboratory renovation and continuing service contracts.