Molecular Cytology Core Facility

分子细胞学核心设施

• 批准号: 9112054
• 负责人: Thomas Phillips
• 金额: $ 13.1万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9112054&HistoricalAwards=false
• 关键词: Molecular; Cytology; Core; Facility

Dr. Thomas Phillips, in conjunction with ten major users, proposes to set up a resource center for immunocytochemistry and in situ hybridization technologies at the University of Missouri. The center would include, and funds are requested for, a confocal fluorescence microscope, a computer graphics system for three-dimensional reconstruction of confocal microscope images, a low-light microscope and camera for fluorescence studies, and an associated image processing system for ration imaging of indicator dyes to determine cellular pH or ion concentrations. The center will incorporate some already existing equipment: a transmission electron microscope, microtomes and a cryostat. This facility would complement existing campus-wide cores for monoclonal antibody production and oligonucleotide synthesis. Various units within the University will provide 50% of the capital costs, long-term salary support for staff, substantial existing equipment, and funding for laboratory renovation and continuing service contracts.

托马斯菲利普斯博士，与十个主要用户， 建议建立免疫细胞化学资源中心， 原位杂交技术， 密苏里州。 该中心将包括，并要求资金， 共聚焦荧光显微镜，计算机图形系统 用于共焦显微镜的三维重建 低光显微镜和荧光照相机 研究，以及相关的图像处理系统， 指示剂染料成像以确定细胞pH或离子 浓度的 该中心将纳入一些已经 现有设备：透射电子显微镜， 切片机和低温恒温器 该设施将补充 现有的校园范围内的单克隆抗体生产核心， 寡核苷酸合成。 大学内各单位 将提供50%的资金成本，长期工资支持 人员、大量现有设备和资金， 实验室翻修和连续服务合同。