Measurement and Characterization of DOM Pools Using EEM Spectroscopy and HPLC

使用 EEM 光谱和 HPLC 测量和表征 DOM 池

• 批准号: 9116497
• 负责人: Arthur Nowell
• 金额: $ 8.68万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-11-15 至 1992-09-01
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9116497&HistoricalAwards=false
• 关键词: Measurement; Characterization; DOM; Pools; Using

The major pool of dissolved organic carbon (DOC) in the ocean is an order of magnitude larger than the particulate organic carbon (POC) pool, and twice as large as the atmospheric pool of CO2, yet as much as 90% of DOC remains uncharacterized as to specific chemical identity. One component of this uncharacterized pool is known as "humic substance" or "gelbstoff", two non-specific terms for a complex mixture of compounds having a yellow color and a blue fluorescence. The way in which these compounds have been defined and analyzed in the past has resulted in much confusion and a lack of insight regarding their nature, distribution, and cycling in the ocean. Recent developments in fluorescent spectroscopy and high performance liquid chromatography show promising results when applied to the study of gelbstoff in seawater. Three-dimensional excitation-emission matrix (EEM) analysis of unconcentrated samples has shown for the first time the existence of four different fluorophores in seawater, each of which exhibits characteristic properties and distributions. One of these fluorophores may be indicative of a protein-like, bioreactive substance. Ion-pairing HPLC can resolve gelbstoff into up to twenty individual components. Application of these new technologies in tandem to the analysis of dissolved organic matter in seawater can be used to trace different components of the DOC, thereby leading to an improved understanding of the oceanic carbon cycle.

海洋中溶解有机碳（DOC）的主要来源是 比颗粒有机碳大一个数量级 (POC)是大气中二氧化碳总量的两倍， 然而，多达90%的DOC仍然没有表征， 化学特性 这个未定性的游泳池的一个组成部分是 被称为“腐殖物质”或“gelbstoff”，两个非特定术语 对于具有黄色的化合物的复杂混合物， 蓝色荧光。 这些化合物被 在过去的定义和分析中， 缺乏对它们的性质、分布和 在海洋中骑自行车。 荧光材料的最新发展 光谱和高效液相色谱显示 有希望的结果时，适用于研究gelbstoff在 海水 三维激发发射矩阵 对未浓缩样本的分析首次表明， 海水中存在四种不同的荧光团， 其表现出特征性质和分布。 一 可以指示蛋白质样， 生物活性物质 离子对高效液相色谱法可分离Gelbstoff 多达20个独立的组件。 应用该等 溶解有机物分析的新技术 海水中的物质可以用来追踪 DOC，从而提高对 海洋碳循环