Proteins Catalysts of Fermentation Respiration and Photosynthesis in Cyanobacteria and Algae

蓝藻和藻类发酵呼吸和光合作用的蛋白质催化剂

• 批准号: 9117600
• 负责人: David Krogmann
• 金额: $ 27万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1997-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9117600&HistoricalAwards=false
• 关键词: Proteins; Catalysts; Fermentation; Respiration; Photosynthesis

Studies of proteins involved in photosynthesis point to an important pathway of fermentation which permits survival of cyanobacteria and algae under dark, anaerobic conditions. Studies of the soluble, low potential cytochrome c which is abundant in some cyanobacteria and algae suggest that this cytochrome links ferredoxin to hydrogenase in supporting the fermentation of carbohydrate reserves. The hypothesis of cytochrome function to be tested is based on data from enzymology and cell physiology. It must be confirmed by the techniques of gene deletion. Studies of the chemistry of catalyst interaction and of regulation of expression of the genes involved are proposed. Different sets of catalysts of this pathway were present in different compartments of the cell. The structure and function of these proteins will be studied. There is evidence that a carotenoid protein may function to protect cyanobacteria from photodestruction in bright light. Experiments are proposed to extend and clarify this hypothesis. Cytochrome c6 may be a catalyst of both photosynthetic and respiratory electron transport. We have found three isoforms of this catalyst. The PI will study the chemical basis for these forms and look for roles in distinct catalytic functions or in targeting to different compartments of the cell.

对参与光合作用的蛋白质的研究表明， 重要的发酵途径，使生存的 蓝藻和藻类在黑暗的厌氧条件下。 研究 可溶性的，低电位的细胞色素c， 一些蓝细菌和藻类表明这种细胞色素连接 铁氧还蛋白转化为氢化酶， 碳水化合物储备 细胞色素功能假说 测试是基于酶学和细胞生理学的数据。 这必须通过基因缺失技术来证实。 研究 催化剂相互作用的化学反应和调节 所涉及的基因的表达。 不同组的 该途径的催化剂存在于不同的隔室中 的细胞。 这些蛋白质的结构和功能将是 研究了 有证据表明，类胡萝卜素蛋白可能起到保护作用， 蓝细菌在强光下的光破坏。 实验 提出了扩展和澄清这一假设。 细胞色素c6可能是光合作用的催化剂， 呼吸电子传递 我们已经发现了三种异构体 这个催化剂。 PI将研究这些的化学基础 形式并寻找在不同催化功能或 靶向细胞的不同区室。