Photoregulated Protein Kinases in Plants

植物中的光调节蛋白激酶

• 批准号: 9118226
• 负责人: John Watson
• 金额: $ 15.81万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1995-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118226&HistoricalAwards=false
• 关键词: Photoregulated; Protein; Kinases; Plants

Protein kinases are ubiquitous enzymes that qare frequently involved in signal transduction processes. The major focus of the proposed work is to elucidate the roles that protein kinases may play in photoregulated leaf development in plants. A suite of partial cDNA clones from the garden pea have been sequenced and found to encode protein serine/threonine kinase homologs. Messenger RNA levels corresponding to the five cDNA clones accumulate differentially when pea seedlings are exposed to white light. Experiments are proposed to understand the photoregulated expression of these putative kinase genes at the RNA and protein level. Full-length cDNA clones will be isolated and sequenced in order to understand more fully the structure of the putative kinases. The photoreceptor system(s) controlling mRNA accumulation and gene transcription will be determined. The polypeptides encoded by the pea cDNA clones will be isolated after expression in bacteria and used to generate antibodies. The antibodies will be used to study the accumulation, distribution, and biochemical properties of the protein kinases. Transgenic tobacco plants expressing the pea cDNAs will be produced in order to understand the potential role these enzymes play in the transduction of light signals.

蛋白激酶是一种普遍存在的酶，经常参与信号转导过程。本文的主要工作重点是阐明蛋白激酶在植物叶片光调节发育中可能发挥的作用。一组来自豌豆的部分cDNA克隆已被测序，并发现编码蛋白丝氨酸/苏氨酸激酶同源物。当豌豆幼苗暴露在白光下时，5个cDNA克隆对应的信使RNA水平积累不同。我们建议通过实验来了解这些假定的激酶基因在RNA和蛋白质水平上的光调控表达。全长cDNA克隆将被分离和测序，以便更充分地了解推定的激酶的结构。将确定控制mRNA积累和基因转录的光感受器系统。由豌豆cDNA克隆编码的多肽将在细菌中表达后被分离出来并用于产生抗体。这些抗体将用于研究蛋白激酶的积累、分布和生化特性。为了了解这些酶在光信号转导中的潜在作用，将产生表达豌豆cdna的转基因烟草植株。