Research on the Conformation of Protein Molecules

芬恩博士将使用一种相对较新的技术， 光谱法来研究大分子的构象。 具体而言，他将：（1）获得非- 氨基酸分子间的共价力 光谱法测量所需的能量， 解离ES电离产生的小团簇;（2）获得 蛋白质表面积的实验信息 构象离子，并研究疏水性和亲水性 表面不均匀性。 虽然重点将放在 在溶液中的蛋白质上， 因为它们可以提供有用的视角。 %%% 蛋白质分子的构象或“三级结构” 决定了它们的生物活性， 在生命系统中的许多化学过程中。 因此，理解任何一个人的行为都是至关重要的。 蛋白质分子它的活性构型是什么沿着以及为什么 以及它是如何产生的。 这些研究的副产品 将是一些关于溶质离子被 由带电液滴形成。

Dr. Fenn will use a relatively new technique of Electrospray Mass spectrometry to study the conformation of macromolecules. Specifically, he will: (1) obtain experimental data on the non- covalent forces between amino acid molecules by using tandem mass spectrometry methods to measure the energies required to dissociate small clusters produced by ES ionization; (2) obtain experimental information on the surface area of a protein conformer ion and to investigate the hydrophobic and hydrophilic heterogeneity of that surface. Although emphasis will be placed on proteins in solution, other kinds of polymers will be considered because they may provide useful perspectives. %%% The conformation or "tertiary structure" of protein molecules determines their biological activity and so is a governing factor in many if not most of the chemical processes in living systems. Consequently, it is of vital importance to understand for any protein molecule what its active configuration is along with why and how it comes about. A hoped-for byproduct of these studies will be some insight on mechanisms by which solute ions are formed from charged droplets.